ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System: Analytical Performance for a Mineralocorticoid
For in vitro diagnostic use. Not available in all countries.
Introduction
The Waters ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System enables the quantification of organic compounds in human biological liquid matrices.
This document describes a test of the analytical performance of the ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System for the analysis of aldosterone in plasma.
Experimental
The ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System was controlled by MassLynx IVD Software (v4.1) and the data processed using the TargetLynx Application Manager. Calibrators and Quality Controls were prepared by spiking commercially available reference material in stripped serum and the samples were processed using the following conditions:
Sample Preparation Conditions
200 μL sample was processed with ZnSO4/methanol, diluted, and centrifuged. Samples were loaded onto Oasis MAX μElution plates, washed, and eluted prior to analysis.
LC Conditions
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Column: |
CORTECS UPLC C18 1.6 μm, 2.1 mm × 100 mm |
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Mobile phase A: |
Water |
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Mobile phase B: |
Methanol |
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Flow rate: |
0.4 mL/min |
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Gradient: |
40% B over one minute, 40–60% B over one minute, 60% for 0.3 minutes, 95% B for 0.5 minutes |
MS Conditions
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Resolution: |
MS1 (0.75 FWHM), MS2 (0.5 FWHM) |
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Acquisition mode: |
MRM |
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Polarity: |
ESI (-) |
Results and Discussion
Performance characteristics of aldosterone using the ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System is shown in Table 1. Analytical sensitivity of the system for analyzing extracted aldosterone plasma samples is illustrated in Figure 1.
Note: To convert SI units to conventional mass units divide by 2.774 for aldosterone (pmol/L to pg/mL).
Conclusion
The Waters ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System has demonstrated the capability to deliver analytical sensitivity and precision for the analysis of aldosterone in plasma.
Disclaimer
The analytical performance data presented here is for illustrative purposes only. Waters does not recommend or suggest analysis of the analytes described herein. These data are intended solely to demonstrate the performance capabilities of the system for analytes representative of those commonly analyzed using liquid chromatography and tandem mass spectrometry. Performance in an individual laboratory may differ due to a number of factors, including laboratory methods, materials used, intra-operator technique, and system conditions. This document does not constitute a warranty of merchantability or fitness for any particular purpose, express or implied, including for the testing of the analytes in this analysis.
720006312, August 2018
