GlycoWorks RapiFluor-MS N-Glycan Starter Kit 96 sample

SKU: 176003635
Glycoworks RapiFluor-MS N-Glycan Starter Kit-96 sample


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Product Description

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 ìm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate. It contains materials to process 96 samples in a 4x24 format. The kit contains:

GlycoWorks Deglycosylation Module:

(4) 0.035 ml/vial Rapid PNGaseF Enzyme

(4) 0.25 ml/vial Glycoworks Rapid Buffer

(1) 4 vials of 10 mg/vial RapiGest SF Surfactant

(1) Intact mAb Mass Check Standard *Shipped in a separate box

GlycoWorks RapiFluor-MS Labeling Module:

(4) 23 mg/vial RapiFluor-MS Reagent

(4) 1 ml/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module:

(4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)

(4) 3 ml/vial Sample Diluent (DMF/ACN Mixture)

(1) GlycoWorks HILIC µElution Plate

GlycoWorks Sample Collection Module:

(15) 1 mL tubes for deglycosylation and labeling reaction

(15) 600 ìL tube inserts for SPE eluate collection

(15) Cap strips with slits for direct injection

(1) 1 mL round collection tray

(1) Waste tray

Extras:

(1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081)

(1) ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7μm, 2.1 x 150 mm (186004742)

(1) RapiFluor-MS Glycan Performance Test Standard

This kit includes p/n: 186010878 which is a reduced packaging combination of the RFMS Labelling, Clean-up, and Sample Collection modules. p/n: 186010878 cannot be purchased separately and will only be sold as part of a kit.

Specifications

  • Number of Analysis

    96

  • Type

    Sample Preparation Workflow

  • Packages per kit

    7

  • Separation Mode

    HILIC

  • Detection Method

    MS, MS/MS, UV, HRMS

  • Storage Condition

    Refer to the storage recommendations of each sub-package

  • System Type

    LC-MS

  • Shelf Life

    Refer to the storage recommendations of each sub-package

  • UNSPSC

    41116104

  • Application

    Glycan

  • Brand

    GlycoWorks

  • Product Type

    Application Kits

  • Units per Package

    1 pk

Cold Shipping This part will be shipped cold and shipping and handling fees will be applied to your cart.

Product Support

Documents

Documents



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Glycoworks RapiFluor-MS N-Glycan Starter Kit-96 sample

As the leader in innovations for biotherapeutic analysis, Waters has been dedicated to reimagining the workflow for released N-glycan analysis, leading to the RapiFluor-MS reagent. Featuring the ionization capability needed to maximize information generated by MS detection, this new workflow speeds up deglycosylation and streamlines labeling. With three simple steps that can take as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit enables laboratories to reduce complicated and time-consuming sample preparation.

The unique chemical attributes of RapiFulor-MS provide for increased fluorescence quantification and supreme mass spectral response. In a single label, chromatographers can get valuable information from characterization to routine monitoring.

The GlycoWorks RapiFluor-MS N-Glycan Start Kit contains materials to process 96 samples in a 4x24 format using the appropriate lab equipment. Waters recommends the use of Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 ìm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate. It contains materials to process 96 samples in a 4x24 format. The kit contains:

GlycoWorks Deglycosylation Module:

(4) 0.035 ml/vial Rapid PNGaseF Enzyme

(4) 0.25 ml/vial Glycoworks Rapid Buffer

(1) 4 vials of 10 mg/vial RapiGest SF Surfactant

(1) Intact mAb Mass Check Standard *Shipped in a separate box

GlycoWorks RapiFluor-MS Labeling Module:

(4) 23 mg/vial RapiFluor-MS Reagent

(4) 1 ml/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module:

(4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)

(4) 3 ml/vial Sample Diluent (DMF/ACN Mixture)

(1) GlycoWorks HILIC µElution Plate

GlycoWorks Sample Collection Module:

(15) 1 mL tubes for deglycosylation and labeling reaction

(15) 600 ìL tube inserts for SPE eluate collection

(15) Cap strips with slits for direct injection

(1) 1 mL round collection tray

(1) Waste tray

Extras:

(1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081)

(1) ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7μm, 2.1 x 150 mm (186004742)

(1) RapiFluor-MS Glycan Performance Test Standard

This kit includes p/n: 186010878 which is a reduced packaging combination of the RFMS Labelling, Clean-up, and Sample Collection modules. p/n: 186010878 cannot be purchased separately and will only be sold as part of a kit.","productType":"Application Kits","thumbnailURL":"https://www.waters.com/content/dam/waters/en/Photography/Products/consumables/analytical-standards-and-reagents/glycoworks-rfms-n-glycan-starter-kit-96-sample.jpg.thumb.319.319.png"},{"name":"Glycoworks RapiFluor-MS N-Glycan Kit-96 sample","webCategory":"ApplicationKits","pageUrl":"https://www.waters.com/nextgen/fi/en/shop/application-kits/176003635-glycoworks-rapifluor-ms-n-glycan-starter-kit-96-sample.html","sku":"176003606","message":"

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive.

This core kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, and the GlycoWorks Sample Collection Module. It contains materials to process 96 samples in a 4x24 format. The kit contains:

GlycoWorks Deglycosylation Module: (4) 0.035 ml/vial Rapid PNGaseF Enzyme (4) 0.25 ml/vial Glycoworks Rapid Buffer (1) 4 vials of 10 mg/vial RapiGest SF Surfactant (1) Intact mAb Mass Check Standard *Shipped in a separate box

GlycoWorks RapiFluor-MS Labeling Module: (4) 23 mg/vial RapiFluor-MS Reagent (4) 1 ml/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module: (4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7) (4) 3 ml/vial Sample Diluent (DMF/ACN Mixture) (1) GlycoWorks HILIC µElution Plate

GlycoWorks Sample Collection Module: (15) 1 mL tubes for deglycosylation and labeling reaction (15) 600 µL tube inserts for SPE eluate collection (15) Cap strips with slits for direct injection (1) 1 mL round collection tray (1) Waste tray

This kit includes p/n: 186010878 which is a reduced packaging combination of the RFMS Labelling, Clean-up, and Sample Collection modules. p/n: 186010878 cannot be purchased separately and will only be sold as part of a kit.","productType":"Application Kits","thumbnailURL":"https://www.waters.com/content/dam/waters/en/Photography/Products/skus/reagents/glycoworks-rapifluor-ms-nglycan-kit-96-sample.jpg.thumb.319.319.png"},{"name":"RapiFluor-MS Glycan Performance Test Standard","webCategory":"StandardsReagents","pageUrl":"https://www.waters.com/nextgen/fi/en/shop/application-kits/176003635-glycoworks-rapifluor-ms-n-glycan-starter-kit-96-sample.html","sku":"186007983","message":"The RapiFluor-MS™ Glycan Performance Test Standard is intended to be used as a benchmarking standard and labeled, complex control to compliment the GlycoWorks™ RapiFluor-MS™ N-Glycan Kit solution. Each vial of RapiFluor-MS™ Glycan Performance Test Standard contains the N-glycan content from 30 µg of pooled human IgG (Sigma I4506), or approximately 400 pmoles of RapiFluor-MS labeled, released N-glycans.","productType":"Application Kits","thumbnailURL":"https://www.waters.com/content/dam/waters/en/Photography/Products/skus/reagents/poly-vial-2mL.jpg.thumb.319.319.png"},{"name":"GlycoWorks RapiFluor-MS N-Glycan Refill Kit-24 sample","webCategory":"ApplicationKits","pageUrl":"https://www.waters.com/nextgen/fi/en/shop/application-kits/176003635-glycoworks-rapifluor-ms-n-glycan-starter-kit-96-sample.html","sku":"176003714","message":"

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This refill kit includes the GlycoWorks Deglycosylation Module (186008841) and the GlycoWorks Labeling Module (186008091) shipped together in order to supplement either the 176003712 or 176003713 kits or as extra reagents for the 96 sample kits. It contains materials to process 24 samples in a 3x8 format. The kit contains: GlycoWorks Deglycosylation Module: (1) 0.035 ml/vial Rapid PNGaseF Enzyme (1) 0.25 ml/vial GlycoWorks Rapid Buffer (3) 3 vials of 3 mg/vial RapiGest™ SF Surfactant GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent (3) 1 ml/vial Reagent Solvent (Anhydrous DMF)

","productType":"Application Kits","thumbnailURL":"https://www.waters.com/content/dam/waters/en/brand-assets/thumbnails/icon-application-kits.jpg.thumb.319.319.png"},{"name":"Intact mAb Mass Check Standard","webCategory":"StandardsReagents","pageUrl":"https://www.waters.com/nextgen/fi/en/shop/application-kits/176003635-glycoworks-rapifluor-ms-n-glycan-starter-kit-96-sample.html","sku":"186006552","message":"Analysis of intact proteins by MS is a powerful approach for protein identification and assignment of protein modifications. The Intact mAb Mass Check Standard is a LC/MS standard used as a qualitative tool for intact mass analysis. It is an intact mouse protein purified by Protein-A with a known molecular weight, ideal for higher molecular weight intact mass measurements. It is provided in a Waters Max Recovery Vial PN 18600327c. This standard is designed to be used for routine MS optimization, system setup and performance testing.","productType":"Standards","thumbnailURL":"https://www.waters.com/content/dam/waters/en/Photography/Products/skus/reagents/clear-vial-2mL-black-cap.jpg.thumb.319.319.png"},{"name":"Glycoworks RapiFluor-MS N-Glycan Starter Kit-24 sample","webCategory":"ApplicationKits","pageUrl":"https://www.waters.com/nextgen/fi/en/shop/application-kits/176003635-glycoworks-rapifluor-ms-n-glycan-starter-kit-96-sample.html","sku":"176003712","message":"

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 µm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate. It contains materials to process 24 samples in a 3x8 format. The kit contains:

\n

GlycoWorks Deglycosylation Module:

\n

(1) 0.035 ml/vial Rapid PNGaseF Enzyme

\n

(1) 0.25 ml/vial Glycoworks Rapid Buffer

\n

(1) 3 vials of 3 mg/vial RapiGest™ SF Surfactant

\n

(1) Intact mAb Mass Check Standard *Shipped in a separate box

\n

GlycoWorks RapiFluor-MS Labeling Module:

\n

(3) 9 mg/vial RapiFluor-MS Reagent

\n

(3) 1 mL/vial Reagent Solvent (Anhydrous DMF)

\n

GlycoWorks RFMS Clean-up Module:

\n

(4) 5 mL/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)

\n

(4) 8 mL/vial Sample Diluent (DMF/ACN Mixture)

\n

(1) GlycoWorks HILIC µElution Plate

\n

GlycoWorks Sample Collection Module:

\n

(15) 1 mL tubes for deglycosylation and labeling reaction

\n

(15) 600 µL tube inserts for SPE eluate collection

\n

(15) Cap strips with slits for direct injection

\n

(1) 1 mL round collection tray

\n

(1) Waste tray

\n

Extras:

\n

(1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081)

\n

(1) ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7μm, 2.1 x 150 mm (186004742) 

\n

(1) RapiFluor-MS Glycan Performance Test Standard

\n

This kit includes p/n: 186010922 which is a reduced packaging combination of the RFMS Labelling, Clean-up, and Sample Collection modules. p/n: 186010922 cannot be purchased separately and will only be sold as part of a kit.

","productType":"Application Kits","thumbnailURL":"https://www.waters.com/content/dam/waters/en/Photography/Products/skus/reagents/glycoworks-rapifluor-ms-nglycan-starter-kit-24-sample.jpg.thumb.319.319.png"},{"name":"Waters Ammonium Formate Solution – Glycan Analysis","webCategory":"StandardsReagents","pageUrl":"https://www.waters.com/nextgen/fi/en/shop/application-kits/176003635-glycoworks-rapifluor-ms-n-glycan-starter-kit-96-sample.html","sku":"186007081","message":"This is a concentrated version of the 50mM Ammonium Formate buffer that is recommended to be used with the Biopharm/Glycan system, using the UPLC H-class and the ACQUITY UPLC BEH Glycan Column. This buffer contains 10ml of 5050 mM Ammonium Formate in a Water w/ 3.8% Formic acid matrix. This allows the end user to directly pour the concentrate into a 1L bottle of LCMS grade water for the correct concentration of 50 mM at a pH of 4.4.","productType":"Standards","thumbnailURL":"https://www.waters.com/content/dam/waters/en/Photography/Products/skus/reagents/brown-bottle-15mL-brown-cap.jpg.thumb.319.319.png"}]}