• Application Note

Gradient Chemical Stability Study on XBridge HILIC

Gradient Chemical Stability Study on XBridge HILIC

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the gradient chemical stability study on XBridge HILIC column.

Introduction

The compounds used in this study are-

  1. Uracil
  2. 5-Fluorocytosine
  3. Cytosine
Figure 1. Structures of the compounds used in this study.

Experimental

Test Conditions

Column:

XBridge HILIC, 2.1 x 50 mm, 3.5 μm

Part Number:

186004432

Mobile Phase A:

95:5 acetonitrile:water with 10 mM NH4 + CH3COO- pH 5.5

Mobile Phase B:

50:50 acetonitrile:water with 10 mM NH4 + CH3COO- pH 5.5

Flow Rate:

0.5 mL/min

Injection Volume:

2.0 μL (full loop)

Weak Needle Wash:

95:5 acetonitrile:water

Sample Diluent:

75:25 acetonitrile:methanol

Temperature:

30 °C

Detection:

UV @ 254 nm

Sampling Rate:

40 pts/sec

Time Constant:

0.05

Instrument:

Waters ACQUITY UPLC with TUV detector

Gradient:

Time(min)

Profile

Curve

%A

%B

0

99

1

6

2.0

1

99

6

2.1

99

1

6

2.5

99

1

6

Results and Discussion

WA64080, August 2009

Back To Top