 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates isocratic analysis of acyclovir on Atlantis T3 Columns.
The compound used in this sudy is Acyclovir.
 
            
        | Column: | Atlantis T3, 4.6 x 50 mm, 3 μm | 
| Part number: | 186003726 | 
| Mobile phase A: | H2O | 
| Mobile phase B: | ACN | 
| Mobile phase C: | 100 mM HCOONH4, pH 3.0 | 
| Flow rate: | 1.0 mL/min | 
| Isocratic mobile phase composition: | 87% A; 3% B; 10% C | 
| Injection volume: | 5 μL | 
| Sample: | Acyclovir (10 μg/mL) in H2O | 
| Column temperature: | 30 ˚C | 
| Sample temperature: | 20 ˚C | 
| Detection: | UV @ 254 nm | 
| Sampling rate: | 5 point/sec | 
| Filter response: | 0.2 | 
| Instrument: | Waters Alliance 2695 with 2996 PDA | 
 
            
        WA60213, January 2009