This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of allantoin on ACQUITY UPLC BEH Amide Columns.
 
            
        | Column: | ACQUITY UPLC BEH Amide, 2.1 x 150 mm, 1.7 μm | 
| Part Number: | 186004802 | 
| Isocratic Mobile Phase B: | 90/10 MeCN/H2O | 
| Flow Rate: | 0.2 mL/min | 
| Injection Volume: | 5.0 μL (PLNO) | 
| Sample Concentration: | 100 μg/mL | 
| Sample Diluent: | 90/10 MeCN/H2O | 
| Column Temperature: | 25 °C | 
| Weak Needle Wash: | 95/5 MeCN/H2O | 
| Detection: | UV @ 210 nm | 
| Sampling Rate: | 20 points/sec | 
| Filter Time Constant: | 0.2 | 
| Instrument: | Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector | 
The compound used in this study is:
 
            
        WA60107, June 2009