• Application Note

Spiramycin in Pork

Spiramycin in Pork
  • Waters Corporation
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Pigs eating on a meadow in an organic meat farm

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This method can be used to monitor Spiramycin, a macrolide antibiotic, in pork.

Introduction

World health organizations are concerned about the antibiotics and antibacterials levels in food. This method can be used to monitor Spiramycin, a macrolide antibiotic, in pork.

Experimental

Pretreatment

  1. Weigh 5 g of sample into 25 mL of water. Homogenize.
  2. Centrifuge at 3000 rpm for 10 minutes.
  3. Collect supernatant.
  4. Re-extract pellet with 15 mL 1.2% metaphosphoric acid/ methanol (50:50, v/v).
  5. Vortex.
  6. Centrifuge at 3000 rpm for 10 minutes. Collect supernatant.
  7. Combine both supernatants.
  8. Filter sample using glass fiber filter.

SPE Procedure

SPE Buffer:

Dissolve 8.7 g dipotassium hydrogen phosphate in 1 L of deionized water, adjust to pH 3 with phosphoric acid.

LC Conditions

System:

ACQUITY UPLC

Column:

ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm

Flow rate:

400 μL/min

Mobile phase A:

0.1% formic acid in water

Mobile phase B:

0.1% formic acid in acetonitrile

Gradient

MS Conditions

MS System:

Waters Quattro Premier XE

MRM transitions:

1: 422.50→101.00

2: 422.50→174.10

Ionization mode:

Positive electrospray (ESI+)

Multiple reaction monitoring

Results and Discussion

Representative chromatogram
Representative chromatogram 100 ppb spiramycin spiked sample in pork muscle. 
Recovery results
Recovery results for 100 ppb spiramycin spiked sample in pork muscle. 

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