Multiresidue Analysis of Pesticides in Grain and Beans

This application brief shows the step wise procedure of the Japan Ministry of Health, Labor and Welfare (JPMHLW) Official Method for multi-residue analysis of pesticides for grain and bean samples.

This application brief shows the step wise procedure of the Japan Ministry of Health, Labor and Welfare (JPMHLW) Official Method for multi-residue analysis of pesticides for grain and bean samples.

Pretreatment

1. Extract 10 g sample with 20 mL of water. Add 50 mL of acetonitrile.

2. Homogenize sample. Centrifuge and collect supernatant.

3. Repeat extraction with 20 mL of acetonitrile.

4. Collect acetonitrile layer and dilute to 100 mL with acetonitrile.

5. Sample 20 mL of diluted acetonitrile extract, add 10 g sodium chloride and 20 mL of 0.5 M of phosphate buffer. Shake for 10 minutes.

6. Collect acetonitrile layer for SPE treatment.