EPA Method 532.0 Determination of Phenylurea Compounds in Drinking Water by Solid Phase Extraction and High Performance Liquid Chromatography with UV Detection

This application brief demonstrates the determination of phenylurea compounds in drinking water by solid phase extraction and High Performance Liquid Chromatography with UV detection.

Phenylurea pesticides that were introduced worldwide in the 1950s are used commercially on a wide range of food crops. Subsequently determined to possess a significant toxicological risk, the agricultural runoff of these compounds may be found in drinking water supplies. The United States Environmental Protection Agency (US EPA) requires that drinking water and raw surface water be monitored for the presence of phenylurea pesticides and related compounds using EPA Method 532.0. The European Union (EU) regulation regarding drinking water (EC Directive 98/83/EC), provides a general rule for pesticides and metabolites. This regulation limits the maximum admissible concentration (MAC) at 0.1 ppb for each individual component, with the total concentration not to exceed 0.5 ppb.

Standard Preparation

Pipette 100 μL of AccuStandard mix M-532 and 20 μL mix M-532-SS into 880 μL 1:1 water/acetonitrile (10 ppm analytes plus surrogates).

Eleuent Preparation

A: 25 mM phosphate 

Dissolve 1.7 g of potassium dihydrogen phosphate (KH₂PO₄) and 850 μL phosphoric acid (H₃PO₄) in 100 mL water. Dilute to 1 L, then filter and degas. Verify that the pH is approximately 2.4.

B: Acetonitrile

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