• Application Note

Synthetic Corticosteroids

Synthetic Corticosteroids
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of synthetic corticosteroids.

Introduction

Corticosteroids have potent anti-inflammatory properties, and are used in a wide variety of inflammatory conditions such as arthritis, colitis, asthma, bronchitis, certain skin rashes, and allergic or inflammatory conditions of the nose and eyes.

Experimental

Conditions

Column:

SunFire C8 4.6 x 150 mm, 5 μm

Part number:

186002737

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

20 μg/mL in water; 1 mg/mL stock solution prepared in acetonitrile

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Isocratic Gradient

Compounds

Column:

SunFire C8 4.6 x 100 mm, 3.5 μm

Part number:

186002731

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

20 μg/mL in water; 1 mg/mL stock solution prepared in acetonitrile

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Isocratic Gradient

Results and Discussion

Compounds

USP Tailing

Prednisolone

1.46

Dexamethasone

1.34

Prednisolone 21-acetate

1.32

Betamethasone 17-valerate

1.1

Compounds

USP Tailing

Prednisolone

1.41

Dexamethasone

1.31

Prednisolone 21-acetate

1.22

Betamethasone 17-valerate

1

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