• Application Note

Sulfamethoxazole and Trimethoprim in Serum

Sulfamethoxazole and Trimethoprim in Serum
  • Waters Corporation
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Plasma preparation for centrifuge

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of Sulfamethoxazole and Trimethoprim in serum using Symmetry Columns.

Introduction

Compounds used in this study are:

1. Sulfamerazine (I.S.)

2. Trimethoprim

3. Sulfamethoxazole

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm (p/n:WAT046980)

Guard column:

Sentry guard column, 3.9 x 20 mm, 5 μm

Mobile phase:

25 mM ammonium acetate, pH 5/methanol 82:18 (v/v)

Flow rate:

1.0 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Detection:

UV @ 290 nm

Results and Discussion

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WA31763.159, June 2003

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