• Application Note

Steroids Analysis Using 4.6 x 20 mm Intelligent Speed Separation Columns

Steroids Analysis Using 4.6 x 20 mm Intelligent Speed Separation Columns
  • Waters Corporation
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Red and white steroid capsules, syringe on top

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of steroids. 

Introduction

The compounds used in this study are:

  1. Prednisone
  2. Cortisone 
  3. Corticosterone 
  4. Estradiol 
  5. Estrone 
  6. Progesterone 
Compounds

Experimental

Conditions

Columns:

Atlantis dC18, 4.6 x 20 mm IS 3 µm, (P/N:186002062)

Symmetry C18, 4.6 x 20 mm IS 3.5 µm, (P/N:186002090)

Symmetry Shield RP18, 4.6 x 20 mm IS 3.5 µm, (P/N:186002092)

XTerra MS C18, 4.6 x 20 mm IS 3.5 µm, (P/N:186001891)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Flow rate:

3.0 mL/min

Injection volume:

10 µL

Sample concentration:

20 µg/mL

Temperature:

30 °C

Detection:

UV @ 220 nm

Instrument:

Alliance 2795 with 996 PDA

Gradient

Gradient

Results and Discussion

Results

Featured Products

WA31787.27, June 2003

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