• Application Note

Procainamide in Serum

Procainamide in Serum
  • Waters Corporation
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Plasma serum preparation in syringe

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of Procainamide in serum using SymmetryShield columns.

Introduction

Compounds used in this application brief are Procainamide and Sulfanilamide.

Experimental

HPLC Method

Column:

SymmetryShield RP8, 3.9 x 150 mm, 5 μm (p/n: WAT200655)

Guard Column:

Sentry guard column 3.9 x 20 mm, 5 μm (p/n: WAT200675)

Mobile phase:

20 mM potassium phosphate, pH 3.0/methanol 97:3

Flow rate:

1.0 mL/min

Injection volume:

50 μL of reconstituted porcine serum extract

Temperature:

30° C

Detection:

UV @ 220 nm

Results and Discussion

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WA31763.127, June 2003

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