 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of morphine and its glucuronide metabolites using Oasis HLB extraction method.
Compounds studied in this application brief are:
1. Morphine-D3-glucuronide
2. Morphine-D6-glucuronide
3. Morphine
 
            
        | Column: | SymmetryShield RP8, 3.9 x 150 mm, 5 μm (p/n: WAT200655) | 
| Mobile phase: | 20 mM potassium phosphate, pH 6.4 | 
| Flow rate: | 1.0 mL/min | 
| Injection volume: | 100 μL of porcine serum extract | 
| Detection: | Fluorescence; ex 280 nm, em 355 nm | 
 
            
         
            
         
            
        WA31764.100, June 2003