This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of beclomethasone.
The compounds used in this study are -
| 
                     Compounds  | 
                     USP Tailing Factors  | 
            
|---|---|
| 
                     Beclomethasone Dipropionate  | 
                     1.1  | 
            
| 
                     Testosterone Propionate (I.S.)  | 
                     1.0  | 
            
            
        | 
                     Column:  | 
                     Symmetry C18, 4.6 x 100 mm, 3.5 μm  | 
            
| 
                     Part numbers:  | 
                     WAT066220  | 
            
| 
                     Mobile phase:  | 
                     Acetonitrile/water 65:35  | 
            
| 
                     Flow rate:  | 
                     1.4 mL/min  | 
            
| 
                     Injection volume:  | 
                     15 μL of 0.1 mg/mL of each beclomethasone-dipropionate and testosterone propionate (internal standard)  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
            
        WA31763.35, June 2003