• Application Note

Barbiturates in Human Urine using Oasis Sample Preparation Products

Barbiturates in Human Urine using Oasis Sample Preparation Products
  • Waters Corporation
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For forensic toxicology use only.

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of barbiturates in human urine. 

Introduction

The compounds used in this study are – 

  1. Phenobarbital 
  2. Butabarbital 
  3. Butalbital 
  4. Amobarbital (I.S.) 
  5. Mephobarbital 
  6. Secobarbital 

Experimental

HPLC Method

Column:

Symmetry Shield RP18, 2.1 x 150 mm, 5 µm

Guard column:

Symmetry Shield RP18, 3.9 x 20 mm, 5 µm

Part numbers:

Column - 186000111, Guard - 186000107

Mobile phase:

50 mM potassium phosphate, pH 7.0/acetonitrile 71:29

Flow rate:

1 mL/min

Injection volume:

80 µL urine extract

Temperature:

30 °C

Detection:

UV @ 214 nm (0.350 AUFS)

Results and Discussion

Chromatogram of A) Blank Urine, B) Spiked Urine

Chromatogram of A) Blank Urine, B) Spiked Urine

Featured Products

WA31764.48, June 2003

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