• Application Note

Alkaloids in Goldenseal

Alkaloids in Goldenseal
  • Waters Corporation
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Cup of herbal tea with various herbs

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of alkaloids in goldenseal using Symmetry C18 Columns.

Introduction

Alkaloids in goldenseal is analyzed in this application brief.

Experimental

Sample Preparation

100 mg sample is extracted with 25 mL of ethanol:water (70:30) 

1 mL of the ethanolic extract is diluted 1:3 with water (~ pH 7)

  • Recovery, measured with certified standards at 100 ppm in reagent water, was greater than 85 % for all compounds
  • All analyses gave results within ±35 % of the expected values with the exception of the liquid supplement (see goldenseal analysis)
  • The selective SPE extraction and cleanup procedure provided a convenient analysis of echinacea phenolics in complex matrix such as in herbal tea

HPLC Method

Column:

Symmetry C18, 4.6 x 250 mm, 5 μm (p/n: WAT054275)

Mobile phase A:

0.1% phosphoric acid

Mobile phase B:

Acetonitrile

Flow rate:

1.5 mL/min

Injection volume:

10 μL

Temperature:

35° C

Detection:

UV @ 330 nm

Gradient

Results and Discussion

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WA31763.20, June 2003

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