{"id":6587,"date":"2025-10-06T16:21:47","date_gmt":"2025-10-06T16:21:47","guid":{"rendered":"https:\/\/www.waters.com\/blog\/?p=6587"},"modified":"2025-10-07T14:11:29","modified_gmt":"2025-10-07T14:11:29","slug":"solid-phase-extraction-from-sample-prep-fundamentals-to-best-practices","status":"publish","type":"post","link":"https:\/\/www.waters.com\/blog\/solid-phase-extraction-from-sample-prep-fundamentals-to-best-practices\/","title":{"rendered":"Solid-Phase Extraction: From Sample Prep Fundamentals to Best Practices"},"content":{"rendered":"\n<p class=\"has--font-size\">Chromatography and mass spectrometry sample preparation is one of the most critical (and underrated) steps in analytical chemistry\u2014ensuring samples are clean and concentrated enough for accurate, reproducible analysis. Shortchanging sample preparation can compromise data accuracy, reduce method robustness, and even cause timely downtime of expensive instrumentation when it matters most.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-6587_5a1f7a-80 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-6587_5a1f7a-80 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-6587_5a1f7a-80\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>What Are the Challenges in Sample Preparation?<\/strong><\/h2>\n\n\n\n<p>The importance of sample preparation and cleaning up your samples comes down to one major challenge: matrix interferences. These interferences can introduce data variability, compromise method reliability, and cause problematic issues with instrumentation\u2014 especially in LC-MS workflows. Cleaning up your samples provides the added benefit of increased sensitivity by concentrating analytes of interest, improving the accuracy and precision of analytical results, and removing interfering substances.<a id=\"_msocom_1\"><\/a><\/p>\n\n\n<style>.kb-image6587_c2c420-63 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image6587_c2c420-63\"><figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"975\" height=\"365\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/challenges-in-sample-preparation-image.png\" alt=\"challenges in sample preparation image\" class=\"kb-img wp-image-6590\" srcset=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/challenges-in-sample-preparation-image.png 975w, https:\/\/www.waters.com\/blog\/wp-content\/uploads\/challenges-in-sample-preparation-image-300x112.png 300w, https:\/\/www.waters.com\/blog\/wp-content\/uploads\/challenges-in-sample-preparation-image-768x288.png 768w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><\/figure><\/div>\n\n\n\n<p>There are many sample preparation techniques, ranging from simple methods like filtration and protein precipitation to more selective and sensitive techniques like solid-phase extraction (SPE), which produces the cleanest extracts. While there are many sample preparation techniques, SPE stands out for its ability to deliver cleaner extracts, reduce matrix interferences, and improve sensitivity, especially in challenging applications. Let\u2019s take a closer look.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-6587_8c3430-86 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-6587_8c3430-86 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-6587_8c3430-86\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>How Does Solid-Phase Extraction Work?<\/strong><\/h2>\n\n\n\n<p>SPE works by passing a liquid sample through a solid adsorbent material (sorbent) that retains the analytes of interest by selecting appropriate solvents and sorbents selective per the analyte. Different sorbent chemistries, such as <strong>silica-based, polymer-based<\/strong>, and <strong>ion-exchange <\/strong>materials, are used depending on the nature of the analytes and the sample matrix. &nbsp;<strong>&nbsp;<\/strong><\/p>\n\n\n\n<p>Common SPE protocols typically work in one of two ways:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Load-wash-elute, <\/strong>which consists of loading the sample, washing away interferences, and eluting, or<\/li>\n\n\n\n<li><strong>pass-through,<\/strong> where interferences are captured in the sorbent and cleaned analytes pass through.<\/li>\n<\/ul>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-6587_afd724-16 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-6587_afd724-16 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-6587_afd724-16\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Choosing Your SPE Sorbent<\/strong><\/h2>\n\n\n\n<p>At Waters, we provide a diverse range of <a href=\"https:\/\/www.waters.com\/nextgen\/global\/products\/sample-preparation\/sep-pak-sample-extraction-products.html\">Sep-Pak Sorbents<\/a>, including many silica-based options designed to help you tackle even the most complex challenges in the lab. <a href=\"https:\/\/www.waters.com\/nextgen\/global\/products\/sample-preparation\/oasis-sample-extraction-products.html\">Oasis Sorbents<\/a> are polymeric sorbents, all based on the backbone of Oasis HLB, each designed to handle different types of analytes and sample matrices with simplified protocols.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Oasis HLB<\/strong> <strong>Sorbent <\/strong>is a hydrophilic-lipophilic balanced sorbent that provides high capacity for acids, bases, and neutrals, making it ideal for a wide range of applications.<\/li>\n\n\n\n<li><strong>Oasis PRiME HLB<\/strong> <strong>Sorbent <\/strong>adds additional capabilities to further remove complex matrix interferences, like phospholipids and salts, when preparing a sample for LC-MS analysis.<\/li>\n\n\n\n<li><strong>Oasis Mixed-Mode Ion Exchange Sorbents<\/strong> (MM-IEX, MCX, MAX, WCX, WAX) offer the highest level of specificity and sensitivity for analytes demanding more selectivity.<\/li>\n<\/ul>\n\n\n<style>.kb-image6587_de858e-92.kb-image-is-ratio-size, .kb-image6587_de858e-92 .kb-image-is-ratio-size{max-width:457px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image6587_de858e-92.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image6587_de858e-92 .kb-image-is-ratio-size{align-self:unset;}.kb-image6587_de858e-92 figure{max-width:457px;}.kb-image6587_de858e-92 .image-is-svg, .kb-image6587_de858e-92 .image-is-svg img{width:100%;}.kb-image6587_de858e-92 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image6587_de858e-92\"><figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"827\" height=\"698\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/oasis-family.png\" alt=\"Oasis product family\" class=\"kb-img wp-image-6591\" srcset=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/oasis-family.png 827w, https:\/\/www.waters.com\/blog\/wp-content\/uploads\/oasis-family-300x253.png 300w, https:\/\/www.waters.com\/blog\/wp-content\/uploads\/oasis-family-768x648.png 768w\" sizes=\"(max-width: 827px) 100vw, 827px\" \/><\/figure><\/div>\n\n\n\n<p>SPE is widely used in various fields, including environmental analysis, pharmaceuticals, food safety, and clinical diagnostics. Routine sorbents used in SPE include Oasis HLB, MM-IEX, C18, C8, and Silica Sorbents, each selected based on specific application and analyte properties. Some unique examples include using WAX sorbents for PFAS due to the ability to bind to the acidic nature of many PFAS, MCX for basic drugs and tryptic peptides, or even Sep-Pak PSA Sorbent for fatty acid cleanup.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-6587_349135-52 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-6587_349135-52 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-6587_349135-52\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Choosing Your SPE Device Format and Sample Processing Options<\/strong><\/h2>\n\n\n\n<p>Selecting the appropriate device format, such as cartridges, 96-well plates, or \u00b5Elution Plates, depends on the sample volume, sample throughput requirements, and the nature of the analytes.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Easy sample prep of one individual sample &#8211;<\/strong> A syringe-based plus-style cartridge can be used for quick cleanup of a sample, such as potentially with food<\/li>\n\n\n\n<li><strong>Large sample batch size &#8211;<\/strong> 96-well options can increase efficiency, such as with bioanalytical assays<\/li>\n\n\n\n<li><strong>Peptide samples <\/strong>&#8211; Peptides could undergo non-specific binding, reducing sensitivity. \u00b5Elution Plates provide additional concentration to increase sensitivity with a unique design that minimizes analyte loss attributable to sticking to the walls of collection plates.<\/li>\n\n\n\n<li><strong>Versatile sample prep <\/strong>\u2013 Traditional vacuum cartridges are a widely used format across all industries. They are well-suited for processing individual samples or small batches, with a variety of sorbent capacities to fit different analytical needs.<\/li>\n<\/ul>\n\n\n\n<p>Proper vial selection for labs is also a crucial aspect of sample preparation. Sample processing options include manual, semi-automated, and fully automated systems, each offering different levels of efficiency and convenience.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-6587_b82d88-af .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-6587_b82d88-af .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-6587_b82d88-af\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>How to Evaluate Your SPE Protocol<\/strong>&nbsp;<\/h2>\n\n\n\n<p>Evaluating your SPE protocol involves measuring three key parameters to determine success.&nbsp;<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>% recovery<\/strong>: The percentage of analyte recovered from the sample.<\/li>\n\n\n\n<li><strong>Matrix effect<\/strong>: The impact of other substances in the sample on the analyte&#8217;s detection.<\/li>\n\n\n\n<li><strong>Mass balance<\/strong>: The total amount of analyte accounted for in the extraction process.<\/li>\n<\/ul>\n\n\n\n<p>If recovery is low, matrix effects high, and reproducibility poor, you may need to troubleshoot the protocol. This involves adjusting the sorbent type, elution solvents, and sample loading conditions. With method optimization, you can ensure that your SPE protocol is robust and reliable for routine use.<a id=\"_msocom_1\"><\/a><\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-6587_760a89-fe .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-6587_760a89-fe .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-6587_760a89-fe\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Getting Started with SPE<\/strong><\/h2>\n\n\n\n<p>Solid-phase extraction is a powerful sample preparation technique, offering versatility and efficiency in various analytical applications. Waters comprehensive line of <a href=\"https:\/\/www.waters.com\/nextgen\/global\/search.html?category=Shop&amp;content_type=samplepreparationfiltration&amp;enableHL=true&amp;keyword=%2A%3A%2A&amp;multiselect=true&amp;page=1&amp;rows=12&amp;sort=most-recent&amp;facet=brand_facet:Oasis\">Oasis<\/a> and <a href=\"https:\/\/www.waters.com\/nextgen\/global\/search.html?category=Shop&amp;content_type=samplepreparationfiltration&amp;enableHL=true&amp;keyword=%2A%3A%2A&amp;multiselect=true&amp;page=1&amp;rows=12&amp;sort=most-recent&amp;facet=brand_facet:Sep-Pak\">Sep-Pak<\/a> SPE Products provide unmatched purity, consistency, and quality, making them the preferred choice for analytical chemists.<\/p>\n\n\n\n<p>For more information on how to optimize your SPE protocols and to explore our full range of SPE products, including our sample prep kits and vials for labs, visit our <a href=\"https:\/\/www.waters.com\/nextgen\/global\/products\/sample-preparation.html\">page on sample preparation<\/a>. For more on getting started with SPE, check out our <a href=\"https:\/\/www.waters.com\/nextgen\/global\/education\/primers\/beginner-s-guide-to-spe.html?srsltid=AfmBOorbMIHK5uKVwAS5UbVyDc-2tOp1gNo9Ltd4KUOdDj0n7-GQeZPz\">Beginner\u2019s Guide to SPE<\/a> or watch our <a href=\"https:\/\/pages.waters.com\/2024-08-Chemistry-CLA-SPE-Back-To-Basics-Webinar.html\">recent webinar<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chromatography and mass spectrometry sample preparation is one of the most critical (and underrated) steps in analytical chemistry\u2014ensuring samples are clean and concentrated enough for accurate, reproducible analysis. Shortchanging sample preparation can compromise data accuracy, reduce method robustness, and even cause timely downtime of expensive instrumentation when it matters most. What Are the Challenges in&#8230;<\/p>\n","protected":false},"author":170,"featured_media":6089,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"none","_seopress_titles_title":"%%post_title%% %%sep%% %%sitetitle%%","_seopress_titles_desc":"Ensure samples are clean and concentrated enough for accurate, reproducible LC & MS analysis with this blog on sample prep & SPE best practices.","_seopress_robots_index":"","_seopress_analysis_target_kw":"sample preparation,solid-phase extraction,spe,sample prep,oasis,oasis sample preparation,oasis spe,sample prep best practices,sample preparation best practices","_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[599,22],"tags":[104,24,711,712,704,710],"class_list":["post-6587","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-featured","category-technology","tag-liquid-chromatography-lc","tag-mass-spectrometry-ms","tag-oasis","tag-oasis-spe","tag-sample-preparation","tag-solid-phase-extraction"],"acf":[],"taxonomy_info":{"category":[{"value":599,"label":"Featured"},{"value":22,"label":"Technology"}],"post_tag":[{"value":104,"label":"liquid chromatography (LC)"},{"value":24,"label":"mass spectrometry (MS)"},{"value":711,"label":"oasis"},{"value":712,"label":"oasis spe"},{"value":704,"label":"sample preparation"},{"value":710,"label":"solid-phase extraction"}]},"featured_image_src_large":["https:\/\/www.waters.com\/blog\/wp-content\/uploads\/adobestock_233949972-1024x576.jpeg",1024,576,true],"author_info":{"display_name":"Chelsea Plummer","author_link":"https:\/\/www.waters.com\/blog\/author\/cplummer\/"},"comment_info":"","category_info":[{"term_id":599,"name":"Featured","slug":"featured","term_group":0,"term_taxonomy_id":599,"taxonomy":"category","description":"","parent":0,"count":30,"filter":"raw","term_order":"0","cat_ID":599,"category_count":30,"category_description":"","cat_name":"Featured","category_nicename":"featured","category_parent":0},{"term_id":22,"name":"Technology","slug":"technology","term_group":0,"term_taxonomy_id":22,"taxonomy":"category","description":"Our expertise drives innovation, development, and\u00a0application of informatics and software that continue to enable leading pharmaceutical, environmental, food &amp; beverage and chemical materials organizations to accelerate decision-making, improve laboratory effectiveness, and get products to market faster.","parent":0,"count":115,"filter":"raw","term_order":"0","cat_ID":22,"category_count":115,"category_description":"Our expertise drives innovation, development, and\u00a0application of informatics and software that continue to enable leading pharmaceutical, environmental, food &amp; beverage and chemical materials organizations to accelerate decision-making, improve laboratory effectiveness, and get products to market faster.","cat_name":"Technology","category_nicename":"technology","category_parent":0}],"tag_info":[{"term_id":104,"name":"liquid chromatography (LC)","slug":"liquid-chromatography-lc","term_group":0,"term_taxonomy_id":104,"taxonomy":"post_tag","description":"","parent":0,"count":25,"filter":"raw","term_order":"0"},{"term_id":24,"name":"mass spectrometry (MS)","slug":"mass-spectrometry-ms","term_group":0,"term_taxonomy_id":24,"taxonomy":"post_tag","description":"","parent":0,"count":61,"filter":"raw","term_order":"0"},{"term_id":711,"name":"oasis","slug":"oasis","term_group":0,"term_taxonomy_id":711,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","term_order":"0"},{"term_id":712,"name":"oasis spe","slug":"oasis-spe","term_group":0,"term_taxonomy_id":712,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","term_order":"0"},{"term_id":704,"name":"sample preparation","slug":"sample-preparation","term_group":0,"term_taxonomy_id":704,"taxonomy":"post_tag","description":"","parent":0,"count":2,"filter":"raw","term_order":"0"},{"term_id":710,"name":"solid-phase extraction","slug":"solid-phase-extraction","term_group":0,"term_taxonomy_id":710,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","term_order":"0"}],"_links":{"self":[{"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/posts\/6587","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/users\/170"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/comments?post=6587"}],"version-history":[{"count":0,"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/posts\/6587\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/media\/6089"}],"wp:attachment":[{"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/media?parent=6587"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/categories?post=6587"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waters.com\/blog\/wp-json\/wp\/v2\/tags?post=6587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}