{"id":4539,"date":"2017-08-03T15:20:00","date_gmt":"2017-08-03T15:20:00","guid":{"rendered":"https:\/\/www.waters.com\/blog\/mastering-polar-compound-retention\/"},"modified":"2023-06-19T19:30:57","modified_gmt":"2023-06-19T19:30:57","slug":"mastering-polar-compound-retention","status":"publish","type":"post","link":"https:\/\/www.waters.com\/blog\/mastering-polar-compound-retention\/","title":{"rendered":"Infographic: What&#8217;s the Best Column for Polar Compound Retention?"},"content":{"rendered":"<h2 style=\"text-align: left;\">How to choose the best stationary phase for polar compounds<\/h2>\n<p>Retaining and separating highly polar, water-soluble organic compounds has historically been a challenge for separation scientists who prefer reversed-phase chromatography as their technique of choice. There are many reasons why a conventional RP columns are not ideal for polar compound retention. However, let\u2019s not dwell on the problems of the past; the development of <a href=\"https:\/\/www.waters.com\/waters\/T3-Columns\/nav.htm?cid=134914589\">T3 bonded particles<\/a> have changed that!<\/p>\n<blockquote><p><a href=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/t3-1.png\"><img decoding=\"async\" class=\"size-full wp-image-3544 alignleft\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/t3-1.png\" alt=\"\" width=\"129\" height=\"100\"><\/a>T3 Particle Technology was developed using optimized C<sub>18&nbsp;<\/sub>ligands and proprietary end-capping to create the ideal column characteristics for polar compound retention.<\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<p>Available in three stationary phases based on unique particle substrates, CORTECS Solid-Core T3, ACQUITY UPLC\/XSelect HPLC HSS T3, and Atlantis silica T3 columns provide enhanced polar compound retention and longer column lifetimes in reversed-phase separations. <strong><em>&nbsp;<\/em><\/strong><\/p>\n<p>Our <a href=\"https:\/\/www.waters.com\/waters\/library.htm?cid=134914589&amp;lid=134918805\">infographic <\/a>can help you choose the best stationary phase to master your polar compound retention work.<\/p>\n<p><a href=\"https:\/\/www.waters.com\/waters\/library.htm?cid=134914589&amp;lid=134918805\"><img decoding=\"async\" class=\"aligncenter wp-image-3530\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/720005828en-scaled.jpg\" alt=\"T3 Particle Technology for polar compound retention\" width=\"600\" height=\"1714\"><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>More resources:<\/strong><\/p>\n<ul>\n<li>Download the white paper:&nbsp; <a href=\"https:\/\/www.waters.com\/waters\/library.htm?cid=511436&amp;lid=1546076\">Designing Reversed-Phase Columns for Polar Compound Retention<\/a><\/li>\n<li>Watch our on-demand webinar: <a href=\"https:\/\/www.waters.com\/Jul25\" target=\"_blank\" rel=\"noopener\">Polar Compound Retention &#8211; Understanding What Works, What Doesn\u2019t Work, and What\u2019s New In Reversed-Phase Polar Compound Retention<\/a><\/li>\n<li>Download the <a href=\"https:\/\/www.waters.com\/waters\/library.htm?cid=513211&amp;lid=1528402\">Atlantis Application Notebook<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Retaining and separating highly polar, water-soluble organic compounds has been a challenge for separations scientists who prefer reversed-phase chromatography as their technique of choice. See how Waters has addressed these analytical challenges with our T3 columns.<\/p>\n","protected":false},"author":78,"featured_media":1076,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"Retaining polar compounds has never been easier.  T3 Particle Technology has made reversed-phase chromatography of polar compounds simple.  See how.","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"none","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[{"_seopress_pro_rich_snippets_type":"faq","_seopress_pro_rich_snippets_faq":[{"question":"What's the best chromatography column for polar compound retention?","answer":"T3 chromatography columns are ideal for polar compound retention. T3 Particle Technology was developed using optimized C18 ligands and proprietary end-capping to create the ideal column characteristics for polar compound retention."}]}],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[25],"tags":[161,113,104,26,290],"class_list":["post-4539","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pharmaceutical","tag-columns","tag-hplc","tag-liquid-chromatography-lc","tag-method-development","tag-polar-compounds"],"acf":[],"taxonomy_info":{"category":[{"value":25,"label":"Pharmaceutical"}],"post_tag":[{"value":161,"label":"columns"},{"value":113,"label":"HPLC"},{"value":104,"label":"liquid chromatography (LC)"},{"value":26,"label":"method development"},{"value":290,"label":"polar compounds"}]},"featured_image_src_large":["https:\/\/www.waters.com\/blog\/wp-content\/uploads\/t3-1.png",129,100,false],"author_info":{"display_name":"Kevin Jenkins","author_link":"https:\/\/www.waters.com\/blog\/author\/kjenkins\/"},"comment_info":"","category_info":[{"term_id":25,"name":"Pharmaceutical","slug":"pharmaceutical","term_group":0,"term_taxonomy_id":25,"taxonomy":"category","description":"We bring high-value technologies designed to solve critical, analytical problems. We enable profound discoveries, optimize lab operations and ensure regulatory compliance. We help customers turn global industry challenges into the medicines that offer hope.","parent":0,"count":100,"filter":"raw","term_order":"0","cat_ID":25,"category_count":100,"category_description":"We bring high-value technologies designed to solve critical, analytical problems. We enable profound discoveries, optimize lab operations and ensure regulatory compliance. 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