{"id":4440,"date":"2016-05-16T21:48:00","date_gmt":"2016-05-16T21:48:00","guid":{"rendered":"https:\/\/www.waters.com\/blog\/wrib-2016-diversity-of-platforms-and-approaches-needed-for-bioanalysts\/"},"modified":"2023-07-07T13:04:02","modified_gmt":"2023-07-07T13:04:02","slug":"wrib-2016-diversity-of-platforms-and-approaches-needed-for-bioanalysts","status":"publish","type":"post","link":"https:\/\/www.waters.com\/blog\/wrib-2016-diversity-of-platforms-and-approaches-needed-for-bioanalysts\/","title":{"rendered":"WRIB 2016: Diversity of Platforms and Approaches Needed for Bioanalysts"},"content":{"rendered":"<p>Held April 18\u201322 in Lake Buena Vista, Florida, <a href=\"https:\/\/www.cfabs.org\/10thWRIB-2016Orlando.php\" target=\"_blank\" rel=\"noopener\">WRIB 2016<\/a> was well attended this year, with about 700 attendees. This 10<sup>th<\/sup> Workshop on Recent Issues in Bioanalysis, where regulators and professionals representing pharma\/biopharma, biotech, CRO organizations gather to discuss challenges in method development and emerging approaches, proved as fruitful as years past.<\/p>\n<figure id=\"attachment_1291\" aria-describedby=\"caption-attachment-1291\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/pool.jpg\" rel=\"attachment wp-att-1291\"><img decoding=\"async\" class=\"wp-image-1291 size-medium\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/pool-300x160.jpg\" alt=\"Hilton Orlando pool (photo credit hilton.com)\" width=\"300\" height=\"160\"><\/a><figcaption id=\"caption-attachment-1291\" class=\"wp-caption-text\">The Hilton Orlando (photo: hilton.com)<\/figcaption><\/figure>\n<p>Here are a few high-level takeaways from the main talks:<\/p>\n<ul>\n<li><strong>It\u2019s about the and, not the or<\/strong>: Both ligand binding assays (LBAs) and analysis by chromatography and mass spectrometry (LC-MS) are essential for bioanalysts. One speaker from a top ten pharmaceutical company stated, &#8220;Stop with the &#8216;either\/or&#8217; regarding LBAs and LC-MS. You need both.&#8221;<\/li>\n<li><strong>LC-MS has earned its place<\/strong>: LC-MS was described as &#8220;a must for bioanalysts&#8221; and &#8220;no longer an emerging technology for the bioanalysis of biotherapeutics and biomarkers.&#8221;<\/li>\n<li><strong>Emergence of High Resolution MS<\/strong>: The expanding role of HRMS for targeted quantification and application in a regulated environment was a recurring theme. Dr. Chris Evans from GSK presented data about how QTof technology <a href=\"https:\/\/www.linkedin.com\/pulse\/gsk-50-fold-increase-assay-sensitivity-anthony-g-marcello?trk=prof-post\" target=\"_blank\" rel=\"noopener\">enabled sensitivity (to 1 pg\/mL in matrix) through enhanced selectivity<\/a> (read the details of <a href=\"https:\/\/blog.waters.com\/tof-hrms-rescuing-low-exposure-bioanalysis-applications-from-chemical-noise\" target=\"_blank\" rel=\"noopener\">how Dr. Evans accomplished a validated, sensitive and robust assay with Tof-HRMS<\/a>).<\/li>\n<li><strong>The next challenge<\/strong>: ADAs (<a href=\"https:\/\/www.waters.com\/waters\/en_US\/Antibody-Drug-Conjugate\/nav.htm?cid=134751069\">anti-drug antibodies<\/a>) are causing issues for bioanalysts was a hot topic.<\/li>\n<li><strong>Smaller is better<\/strong>: Microflow applications were touted as a viable option for high-sensitivity needs. Waters\u2019 ionKey\/MS System was recognized by one large pharmaceutical company for its increased sensitivity, lower sample volumes, solvent consumption, ease-of-use, and standardization.\n<ul>\n<li><a href=\"https:\/\/bit.ly\/ionkeyNB\">Application Notebook: Microflow LC-MS<\/a><\/li>\n<li><a href=\"https:\/\/event.on24.com\/eventRegistration\/EventLobbyServlet?target=lobby20.jsp&amp;eventid=1182775&amp;sessionid=1&amp;key=35A534F6338AF5C1CEA4439D9E4684DF&amp;eventuserid=141134265\">Webinar: \u201cThe Versatility of Microflow LC\/MS\u201d<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<figure id=\"attachment_1292\" aria-describedby=\"caption-attachment-1292\" style=\"width: 571px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/wide_wrib.jpg\" rel=\"attachment wp-att-1292\"><img decoding=\"async\" class=\" wp-image-1292\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/wide_wrib-300x86.jpg\" alt=\"Photo from the main room at the Hilton Orlando\" width=\"571\" height=\"164\"><\/a><figcaption id=\"caption-attachment-1292\" class=\"wp-caption-text\">WRIB had a full conference in Orlando.<\/figcaption><\/figure>\n<p>Like the talks, the poster sessions included a variety of bioanalysis techniques and were well attended. There was particularly high engagement near the four Waters protein quantification posters.<\/p>\n<p>With a diversity of options for protein quantification, numerous questions arise regarding the advantages of each approach. &nbsp;Can intact mAbs be quantified using LC-MS? Is reduction and alkylation always needed for a surrogate peptide approach? Are there advantages to using a kit when performing the surrogate peptide method? Does a bottom-up or top-down approach produce higher sensitivity? Download the posters below to address these questions and many others.<\/p>\n<p>Waters&nbsp;presented five posters in total:<\/p>\n<ul>\n<li><a href=\"https:\/\/dmpk.waters.com\/resource\/new-systematic-method-development-quantification-proteins-surrogate-peptide-approach-using\">Systematic Method Development for Quantification of Proteins via the Surrogate Peptide Approach Using LC-MS\/MS, MassLynx, and Skyline<\/a><\/li>\n<li><a href=\"https:\/\/dmpk.waters.com\/resource\/new-development-generic-kit-based-approach-quantifying-protein-therapeutics-biological\">Development of a Generic Kit-Based Approach for Quantifying Protein Therapeutics in Biological Matrices by LC-MS\/MS<\/a><\/li>\n<li><a href=\"https:\/\/dmpk.waters.com\/resource\/new-quantification-intact-protein-biotherapeutics-microfluidic-lc-ms-platform-poster\">Quantification of Intact Protein Biotherapeutics by a Microfluidic LC-MS Platform<\/a><\/li>\n<li><a href=\"https:\/\/dmpk.waters.com\/resource\/new-rapid-simplified-and-highly-efficient-analysis-urinary-thc-and-metabolites-using-novel\">Rapid, Simplified and Highly Efficient Analysis of Urinary THC and Metabolites Using a Novel Reversed-Phase Extraction Sorbent<\/a><\/li>\n<li><a href=\"https:\/\/dmpk.waters.com\/resource\/new-utilizing-lc-ms-tool-box-protein-bioanalysis-poster\">Utilizing the LC-MS Tool Box in Protein Bioanalysis<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_1387\" aria-describedby=\"caption-attachment-1387\" style=\"width: 630px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/wrib-3-pics.png\" rel=\"attachment wp-att-1294\"><img decoding=\"async\" class=\"wp-image-1387\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/wrib-3-pics-300x74.png\" alt=\"\" width=\"630\" height=\"155\"><\/a><figcaption id=\"caption-attachment-1387\" class=\"wp-caption-text\">Left image: Waters DMPK Business Manager Kelly Doering kicks-off the Waters-sponsored opening ceremony. Middle image: The majority of the Waters team at the Waters booth. Right image: Waters scientist Nikunj Tanna presents on protein quantification using Skyline and MassLynx software.<\/figcaption><\/figure>\n<p>The next conference on the travel schedule is ASMS in San Antonio, Texas. Waters is hosting a number of concurrent events including a users meeting, breakfast seminars, oral and poster presentations, and a hospitality suite. Up-to-date information can be found at our <a href=\"https:\/\/wvmc.waters.com\/asms\/\">Waters ASMS website<\/a>. Hope to see you there!<\/p>\n<p>For additional information on Waters bioanalysis applications, please visit&nbsp;<a href=\"https:\/\/dmpk.waters.com\/\">https:\/\/dmpk.waters.com\/<\/a>.<\/p>\n<p><a href=\"https:\/\/dmpk.waters.com\" rel=\"attachment wp-att-1371\"><img decoding=\"async\" class=\"wp-image-1371 aligncenter\" src=\"https:\/\/www.waters.com\/blog\/wp-content\/uploads\/dmpk_tag_image-300x51.jpg\" alt=\"dmpk_tag_image\" width=\"365\" height=\"62\"><\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Held April 18\u201322 in Lake Buena Vista, Florida, WRIB 2016 was well attended this year, with about 700 attendees. This 10th Workshop on Recent Issues in Bioanalysis, where regulators and professionals representing pharma\/biopharma, biotech, CRO organizations gather to discuss challenges in method development and emerging approaches, proved as fruitful as years past. Here are a&#8230;<\/p>\n","protected":false},"author":139,"featured_media":503,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"WRIB 2016: Diversity in Bioanalytical Platforms and Approaches | Waters Blog","_seopress_titles_desc":"The well-attended WRIB 2016, brought together regulators and professionals from various industries to discuss method development and emerging approaches in bioanalysis.","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"none","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[25],"tags":[61,182],"class_list":["post-4440","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pharmaceutical","tag-bioanalysis","tag-workshop-on-recent-issues-in-bioanalysis-wrib"],"acf":[],"taxonomy_info":{"category":[{"value":25,"label":"Pharmaceutical"}],"post_tag":[{"value":61,"label":"bioanalysis"},{"value":182,"label":"Workshop on Recent Issues in Bioanalysis (WRIB)"}]},"featured_image_src_large":["https:\/\/www.waters.com\/blog\/wp-content\/uploads\/wide_wrib.jpg",1024,293,false],"author_info":{"display_name":"Waters Staff","author_link":"https:\/\/www.waters.com\/blog\/author\/waters_staff\/"},"comment_info":"","category_info":[{"term_id":25,"name":"Pharmaceutical","slug":"pharmaceutical","term_group":0,"term_taxonomy_id":25,"taxonomy":"category","description":"We bring high-value technologies designed to solve critical, analytical problems. 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