{"id":4392,"date":"2015-02-09T18:29:00","date_gmt":"2015-02-09T18:29:00","guid":{"rendered":"https:\/\/www.waters.com\/blog\/now-available-peptide-and-protein-bioanalysis-application-notebook\/"},"modified":"2023-07-06T18:00:09","modified_gmt":"2023-07-06T18:00:09","slug":"now-available-peptide-and-protein-bioanalysis-application-notebook","status":"publish","type":"post","link":"https:\/\/www.waters.com\/blog\/now-available-peptide-and-protein-bioanalysis-application-notebook\/","title":{"rendered":"Now Available: Peptide and Protein Bioanalysis Application Notebook"},"content":{"rendered":"

\"P&P<\/a><\/strong><\/p>\n

Peptides and proteins are not small molecules. Why treat them the same?<\/strong><\/p>\n

Analyzing large molecules\u00a0may be one of the greatest challenges that the bioanalyst\u00a0faces at the beginning of the 21st century. Employing LC-MS for the bioanalysis\u00a0of large molecules\u00a0requires the extraction of the target analyte from a matrix\u00a0of biochemically similar peptides and proteins. However, we have seen that\u00a0the most common techniques employed for small molecule extraction, such as\u00a0protein precipitation (PPT) and liquid-liquid extraction (LLE), do not provide the\u00a0recovery, sensitivity, specificity, and assay robustness required for large molecules.\u00a0The LC parameters are very different than those used for small\u00a0molecules. In a addition, writing a multiple reaction monitoring (MRM) method\u00a0presents a unique set of considerations when working with peptides and proteins, including\u00a0the presence of multiple precursors and a range of few to many lower\u00a0abundance fragments.<\/p>\n

To support bioanalysts in their quantification of large molecules, Waters has created a new educational resource,\u00a0the\u00a0Peptide and Protein Bioanalysis Application Notebook<\/i><\/b><\/a>.\u00a0<\/i><\/b><\/i>The compilation includes 21 application notes published between 2009-2014, organized into key areas: general best practices, endogenous analytes, therapeutics, and disease state. A tear-away infographic<\/a> illustrates how and why large molecules must be treated differently than small molecules. The notebook also includes an index of more than 30 featured molecules, which link to the various app notes in which they appear.<\/p>\n

There are a number of new educational resources to\u00a0improve your quantification of large molecules<\/strong><\/p>\n

Download the Peptide and Protein Bioanalysis Application Notebook<\/a>
\nDownload the Peptide and Protein Bioanalysis Infographic<\/a>
\nDownload the Peptide Bioanalysis Solutions Guide<\/a><\/p>\n

Waters\u00ae<\/sup>\u00a0understands the subtleties within each parameter affecting the\u00a0development of robust, reliable, high sensitivity bioanalytical assays for large molecules. In addition to the resources named above, we have developed sample preparation protocols and separation conditions\u00a0that will help you quickly and effectively develop methods for any peptide or protein. We have also built our expertise into the LC-MS software to provide you the\u00a0tools that will enable you to quickly make the transition from small molecule\u00a0to peptide and protein bioanalysis.<\/p>\n","protected":false},"excerpt":{"rendered":"

Peptides and proteins are not small molecules. Why treat them the same? Analyzing large molecules\u00a0may be one of the greatest challenges that the bioanalyst\u00a0faces at the beginning of the 21st century. Employing LC-MS for the bioanalysis\u00a0of large molecules\u00a0requires the extraction of the target analyte from a matrix\u00a0of biochemically similar peptides and proteins. However, we have…<\/p>\n","protected":false},"author":139,"featured_media":362,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"New Release: Peptide and Protein Bioanalysis Application Notebook | Waters Blog","_seopress_titles_desc":"Analyzing large molecules presents unique challenges for bioanalysts. 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