Waters has a solution for your peptide separations.
We offer a wide variety of reversed-phase columns for the analysis and lab-scale isolation of a wide range of peptides (large and small, acidic and basic, hydrophobic and hydrophilic). These unique columns are based on innovative particle technologies that include Waters' Ethylene-Bridged Hybrid (BEH) and Charged Surface Hybrid (CSH™) offerings which are exclusively quality tested with tryptic protein digests to help ensure consistent batch-to-batch and column-to-column performance consistency. Nano, capillary, analytical, and lab-scale preparative columns are available in a wide range of particle sizes (1.7 µm to 10 µm) that address specific peptide application needs. In addition to these premier hybrid-based particle offerings, Waters also offers a series of silica-based, reversed-phase column chemistries that provide different peptide separation selectivities.
Select the right LC column using the following criteria.
BASE Particle Type
Base Particle Type
Waters Peptide CSH C18 Columns expand upon Waters BEH Technology™ in a unique way. The addition of a well-controlled, low level surface charge makes this particle particularly well suited for LC-MS applications using non-TFA mobile phases.
Waters Peptide HSS Columns are designed for separations where silica-based selectivities are desired or if separations are insufficient when using the BEH and CSH columns. This stationary phase provides increased retentivity for peptides.
Waters Peptide BEH C18 Columns provide the most flexibility for the development of UPLC, UHPLC, HPLC, and lab-scale peptide applications ranging from synthetic peptides to complex peptide maps.
BEH (Ethylene-Bridged Hybrid)
CSH (Charged Surface Hybrid)
HSS (High Strength Silica)
Trifunctional C18 ligand, fully end-capped, bonded to Ethylene-Bridged Hybrid (BEH) substrate.
Trifunctional C18 ligand, fully end-capped, bonded to Charged Surface Hybrid (CSH) substrate.
Trifunctional C18 ligand, fully end-capped, bonded to High Strength Silica (HSS) substrate.
Particle Sizes (scalable)
1.7 µm, 3.5 µm, 5 µm , 10 µm
1.7 µm, 2.5 µm, 3.5 µm, 5 µm
1.8 µm, 2.5 µm, 5 µm
Column Quality Control
Method Validation Kits
Columns specifically QC tested with tryptic digests of cytochrome c (P/N 186006371) to ensure column-column consistency with validated methods.
Columns specifically QC tested with tryptic digests of cytochrome c (P/N 186006371) using 0.1% FA-containing eluents for LC-MS applications.
Unlike the hybrid particles, the HSS particles are not specifically QC tested using tryptic digests, but they are still manufactured with strict, quality control tests for batch-to-batch consistency.
ACQUITY UPLC Peptide BEH
ACQUITY UPLC Peptide CSH
ACQUITY UPLC HSS T3 Column
Peptide Columns Debates
A video series of debates from our leading column candidates for reversed-phase peptide separations to help you choose the right columns for your peptide analyses.
LC & LC-MS applications
for peptide analyses.
Peptide Standards and Kits
For the development, validation, and troubleshooting of peptide applications.
Still not sure what you need for your peptide analysis?
I want to learn more about Waters Peptide Columns!