Focused Gradient Calculator     
     
    Start Gradient   Focus Gradient  
System Dwell volume (mL)        
ID (mm)          
Length (mm)          
Particle Size µm          
Low Focusing limit (% organic, Guide: 5-10%)      
High Focusing limit (% organic, Guide: 3-10%)      
  Override Focus Flow Rate  
Flow rate (mL/min)    
Enter Target peak retention time (min)   % Elution of Target Peak    
     
Start Gradient Conditions Focused Gradient Conditions  
  Time % Organic   Time % Organic  
Initial Conditions 0.0   Initial Conditions 0.00  
Hold / Start Separation   Hold / Start Separation  
End Separation / Start Wash   End Separation  
End Wash   Start Wash  
Start Equilibration   End Wash  
End Equilibration   Start Equilibration  
      End Equilibration  
               
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Version date: 17.250 How to Calculate System Dwell Volume © Waters Corporation 2017