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Compare Column
Backpressures & Mass Loads
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LC Column A
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LC Column B
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Inner Diameter
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mm
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mm
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Length
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mm
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mm
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Particle size
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µm
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µm
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Solvent Name
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Flow Rate
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mL / min
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mL / min
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Solvent viscosity (cP)
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Mass Load
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mg
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mg
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Injection volume
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µL
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µL
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Backpressure
(column only)
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psi
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psi
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bar
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bar
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Available column volume (for solvent):
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mL
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mL
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Recommended
Injection volumes
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min
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µL
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µL
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max
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µL
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µL
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Recommended
Flow rates
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min
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mL/min
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mL/min
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max
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mL/min
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mL/min
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Small molecule, RP
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mg
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mg
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Mass
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Small molecule, NP
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mg
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mg
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These are estimates for
loading and will
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Load
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Peptides, RP
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mg
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mg
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vary with sample type
and solvent choice
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Guide:
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Proteins / Peptides, SEC
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mg
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mg
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(RP = Reversed Phase, NP
= Normal Phase)
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Proteins, IEX, isocratic
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mg
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mg
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Proteins, IEX, gradient
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mg
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mg
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Adjust
Backpressure (adjust to take into account
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different
packing densities and system contribution)
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Press reset
to go back to default
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