When considering MS performance claims, be mindful of the small print.
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For greater results, it's got to be Waters. To meet the global call for new, ground-breaking medicines, you need a future-focused DMPK partner who delivers industry-leading, end-to-end solutions. A laboratory technology partner who can help you drive the development of large and small molecules both in bioanalysis and metabolism studies.

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There's a lot of hype out there about needing ultra-high resolution for quantitative analyses.
But the reality about 140,000 is that it's a setting. It doesn't take into consideration the tradeoff in time to achieve that resolution – and that the number gets smaller with increasing m/z.

 

 

Linking mass resolution with quantitative accuracy and precision is both misleading and inaccurate.

  • In many workflows, resolution is a function of both transient time and m/z.
  • In electrospray ionization, sensitivity is a function of ion flux since narrow peaks are preferred.
  • Accurate quantification requires a minimum of 10 spectra across a chromatographic peak.

Waters delivers high resolution time-of-flight mass spectrometers (HRMS) that, by design, have built-in compatibility with fast UPLC separations, no matter what m/z you're looking at.

Consider the molecules that are being analyzed in DMPK labs today, especially biotherapeutics. How many benefit from good chromatography and have a mass greater than 600 daltons?

As an example, how many seconds do you believe it will take your non-Tof based HRMS system to achieve 10 quantitative "ultra-high" resolution scans across peak at m/z 600 or m/z 900? Hint: At 600 m/z, it's greater than 15 seconds. And at 900 m/z, it's 24 seconds!

 

Shine a spotlight on what resolution and sensitivity are really all about.

Discover the truth behind HRMS analyses:


See the difference that true HRMS makes when facing the challenges of peptide bioanalysis: