nanoACQUITY UPLC system features:

• Customize your throughput and condition requirements with comprehensive system components including the nanoACQUITY^® Heating and Trapping Module, the Sample Manager, the Binary Solvent Manager, and the Auxiliary Solvent Manager (optional).
• Monitor system performance with enhanced on-board diagnostics.
• Extend high-sensitivity nano-scale MS analyses: from the Quattro Premier™ XE tandem quadrupole mass spectrometer for routine quantification; to the SYNAPT™ HDMS™ System for cutting edge applications; to the Identity^E High Definition Proteomics System for protein identification.
• Predictive system support with Connections INSIGHT^® remote intelligent services.
• Instrument control and data processing with MassLynx™ Software.
• Easily compatible with third-party MS solutions.
• FlexCart lets you move nanoACQUITY UPLC system from one MS to another.
• Stand-alone nanoACQUITY UPLC console provides easy instrument control.

In this tryptic digest of human cells (n=6 over a 12-hour period), it is possible to see retention time reproducibility usually achieved at analytical scale flow rates. Using shallow gradient conditions, there is a change of 0.3% B/min over two hours. Changes in retention time between comparable samples are due to changes in peptide structure (an indication of changes in protein expression).

In the data below, we see a separation of protein digest standards on columns packed with 3.0 mm particles (top), compared with the same experiment using 1.7 mm BEH particles (bottom). Running the 1.7 mm column produces higher peak capacities, narrower and highly concentrated peaks, and more MS information.

In the nanoACQUITY UPLC System’s Heating and Trapping Module, the analytical column is housed in an extendable arm that delivers analytes to the source of the mass spectrometer. It is contained in a thermally controlled sleeve, which, when combined with the minimized dead volume of the system, leads to improved peak shape, peak resolution, and reproducibility for HPLC and UPLC separations. A trapping column can also be easily added to desalt and pre-concentrate larger sample volumes.

The chromatographic separation of a tryptic digest of 200 fmol yeast enolasse using (A) direct loading and trapping onto the analytical column and (B) sample trapping followed by separation on an analytical column. Both methods demonstrate the ability to efficiently resolve the same peptide components, revealing virtually identical chromatographic profiles.

Binary Solvent Manager

The nanoACQUITY UPLC System’s Binary Solvent Manager (BSM) features responsive flow sensors, optimized tubing, and sophisticated algorithms enable flow rates from 200 nL/min. An automated vent valve facilitates rapid priming and purging and solvent select valves permit alternate solvents for each pump. Two high pressure streams, in 25 µm I.D. tubing, converge in the tee. The system volume from the site of gradient mixing in the tee to the trapping column is <1 µL.