Literature


Description
Oasis PRiME HLB Cartridge for Cleanup of Infant Formula Extracts Prior to UPLC-MS/MS Multiresidue Ve...
Library Number: APNT134898729 | Format: PDF | File Size: 187.94 kB | Year: 2016 | Content Type: Application Notes

In this application note a simple and effective extraction/protein precipitation procedure was applied to the analysis of infant formula.

CASE STUDY 3: Quantitative and Qualitative Analysis of Perfluoroalkyl Substances (PFASs) in Wildlife...
Library Number: APNT134898813 | Format: PDF | File Size: 1449.68 kB | Year: 2016 | Content Type: Application Notes

In this application note, we demonstrate low-level detection and comprehensive data review of fluorinated environmental contaminants in wildlife using HRMS technologies and an integrated scientific information system.

Highly Analytically-sensitive Analysis of Aldosterone in Plasma on the Xevo TQ-XS for Clinical Resea...
Library Number: APNT134897347 | Format: PDF | File Size: 1005.33 kB | Year: 2016 | Content Type: Application Notes

In this technology brief, the Xevo TQ-XS was used to significantly improve the analytical sensitivity and imprecision of a LC-MS/MS method for the analysis of aldosterone in plasma.

Streamline Method Development with Empower 3 MS Peak Tracking
Library Number: APNT134897305 | Format: PDF | File Size: 746.93 kB | Year: 2016 | Content Type: Application Notes

This technology brief demonstrates the use of Empower 3 MS Peak Tracking in the tracking of peak elution during development of a UPLC method for metoclopramide HCl and related substances by mass detection.

Improved Extraction of THC and its Metabolites from Oral Fluid Using Oasis PRiME HLB Solid Phase Ext...
Library Number: APNT134897294 | Format: PDF | File Size: 250.68 kB | Year: 2015 | Content Type: Application Notes

This application note details the extraction and analysis of THC and its major metabolites from oral fluid samples using an Oasis PRiME HLB μElution Plate, followed by UPLC-MS/MS analysis.

Ultimate Resolution HPLC-Based Size Exclusion Chromatography for the Analysis of Small Proteins and ...
Library Number: APNT134841954 | Format: PDF | File Size: 1595.62 kB | Year: 2015 | Content Type: Application Notes

This application note will cover the performance characteristics of this column, designed for the separation of small proteins and peptides, with respect to UPLC method transfer, column-to-column reproducibility, and column stability.

Rapid Screening of Fatty Acids in Oil Supplements Using Ambient Ionization (DART) and Mass Detection
Library Number: APNT134897040 | Format: PDF | File Size: 1509.35 kB | Year: 2016 | Content Type: Application Notes

In this application note, we demonstrate the utility and ease-of-use of DART coupled to single quadrupole mass detection for the identification and rapid authentication of omega-3 and omega-6 polyunsaturated fatty acids in oil supplements.

Rapid Profiling and Authentication of Cinnamon Samples Using Ambient Ionization (DART) and Single Qu...
Library Number: APNT134897051 | Format: PDF | File Size: 1884 kB | Year: 2016 | Content Type: Application Notes

In this application note, we describe a novel approach that employs DART and the ACQUITY QDa Detector for the simple and rapid analysis of cinnamon samples to determine species origin.

Increasing Efficiency of Method Validation for Metoclopramide HCl and Related Substances with Empowe...
Library Number: APNT134808554 | Format: PDF | File Size: 2492.32 kB | Year: 2014 | Content Type: Application Notes

We demonstrate the validation of a UPLC method for linearity, detection and quantitation limits, accuracy, repeatability, intermediate precision, specificity, and robustness using Empower 3 Method Validation Manager.

Simple, Standardized, and Sensitive Quantification of Bevacizumab (Avastin) Using ProteinWorks eXpre...
Library Number: APNT134873721 | Format: PDF | File Size: 1184.69 kB | Year: 2016 | Content Type: Application Notes

This application note describes the fast, sensitive quantification of bevacizumab from rat plasma using the ProteinWorks eXpress Digest Kit and Protocol.

Quantification of the Antibody Drug Conjugate, Trastuzumab Emtansine, and the Monocolonal Antibody, ...
Library Number: APNT134877891 | Format: PDF | File Size: 1347.63 kB | Year: 2016 | Content Type: Application Notes

This application note describes the total mAb quantification of the ADC, ado-trastuzumab emtansine, and the mAb, trastuzumab from rat plasma using the ProteinWorks eXpress Direct Digest Kit and Protocol.

Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer
Library Number: APNT134895769 | Format: PDF | File Size: 1007.76 kB | Year: 2016 | Content Type: Application Notes

The impact of dwell volume & extra-column volume on method transfer is described in this application note.

ACQUITY UPLC PDA Analysis if Biocides (Part 1)
Library Number: APNT134895139 | Format: PDF | File Size: 762.74 kB | Year: 2015 | Content Type: Application Notes

This application note describes a three-minute separation of six biocides using the ACQUITY UPLC PDA System with Empower 3 Software.

Rapid Digestion and Reproducible LC-MS Quantification of Cytochrome C: A Potential Biomarker for Apo...
Library Number: APNT134894215 | Format: PDF | File Size: 477.05 kB | Year: 2016 | Content Type: Application Notes

This application note demonstrates proof of concept for the rapid, accurate and precise quantification of the protein biomarker, cytochrome C.

Process Monitoring of Monoclonal Antibody Subunits for Fast, Critical Quality Attribute Monitoring U...
Library Number: APNT134894031 | Format: PDF | File Size: 742.46 kB | Year: 2016 | Content Type: Application Notes

The scope of the current study is to evaluate the applicability of the ACQUITY QDa Detector for subunit analysis.

Ultra Low-Level Detection of Perfluoroalkyl Substances (PFASs)
Library Number: APNT134893168 | Format: PDF | File Size: 1038.67 kB | Year: 2016 | Content Type: Application Notes

In this technology brief, we describe the detection of routinely monitored PFASs that was performed on the ACQUITY UPLC I-Class System and Xevo TQ-S. Special considerations for the I-Class with flow through needle (FTN) hardware are also detailed.

USP Analysis of Glimepiride on an Alliance HPLC System: Modernization of a USP Method
Library Number: APNT134892351 | Format: PDF | File Size: 247.04 kB | Year: 2016 | Content Type: Application Notes

In this application, the assay for glimepiride was performed according to the USP monograph.

Automated Disulfide Bond Mapping, and Comparing Innovator and Biosimilar mAbs Using UNIFI
Library Number: APNT134892431 | Format: PDF | File Size: 1703.89 kB | Year: 2016 | Content Type: Application Notes

This application note describes a UPLC-MSE analysis of monoclonal antibody (mAb) disulfide bond mapping.

Pressurized Online Pepsin Digestion of mAb IgG2 for Hydrogen Deuterium Exchange Mass Spectrometry
Library Number: APNT134892458 | Format: PDF | File Size: 462.23 kB | Year: 2016 | Content Type: Application Notes

Here we report an improvement in protein digestion efficiency using a high-pressure, sustainable pepsin column – the Enzymate™ Digestion Column.

Analysis of 17-Hydroxyprogesterone in Serum for Clinical Research
Library Number: APNT134892413 | Format: PDF | File Size: 774.36 kB | Year: 2016 | Content Type: Application Notes

This application note describes a clinical research method utilizing Oasis PRiME HLB μElution Plate technology for the extraction of 17-OHP.

Top-down Monoclonal Antibody (mAb) Analysis Using CID and ETD Fragmentation and the UNIFI Scientific...
Library Number: APNT134891412 | Format: PDF | File Size: 4212.07 kB | Year: 2016 | Content Type: Application Notes

This application note demonstrates the acquisition of protein top-down analysis.

An Improved Workflow for DESI Imaging Mass Spectrometry Incorporating Waters High Definition Imaging...
Library Number: APNT134887440 | Format: PDF | File Size: 1468.41 kB | Year: 2016 | Content Type: Application Notes

This technology brief describes a new software acquisition approach which streamlines DESI imaging workflow to maximize data acquisition.

High Definition Imaging Software: Enhanced Visualization and Overlay of Molecular and Optical Images
Library Number: APNT134890471 | Format: PDF | File Size: 3038.61 kB | Year: 2016 | Content Type: Application Notes

This technology brief highlights the new tools for manipulation of ion and optical image overlays in Waters High Definition Imaging (HDI®) Software version 1.4. This includes tools for alignment and overlay blending modes.

Streamlining Compound Isolation Automatically with UPLC to Prep Chromatography using Mass-Directed A...
Library Number: APNT134890483 | Format: PDF | File Size: 1511.93 kB | Year: 2016 | Content Type: Application Notes

This study illustrates how UPLC system can be dedicated for compound screening and fraction analysis.

Coupling Mass Detection with UV to Improve Method Sensitivity for Esters of Benzenesulfonic Acid in ...
Library Number: APNT134890242 | Format: PDF | File Size: 1234.86 kB | Year: 2016 | Content Type: Application Notes

In this application note, we present a robust and quick UPLC method for analysis of methyl, ethyl, and isopropyl esters of benzenesulfonic acid.