Literature


Description
Ultimate Resolution HPLC-Based Size Exclusion Chromatography for the Analysis of Small Proteins and ...
Library Number: APNT134841954 | Format: PDF | File Size: 1595.62 kB | Year: 2015 | Content Type: Application Notes

This application note will cover the performance characteristics of this column, designed for the separation of small proteins and peptides, with respect to UPLC method transfer, column-to-column reproducibility, and column stability.

Applying a Novel Glycan Tagging Reagent, Rapi Fluor-MS, and an Integrated UPLC-FLR/QTof MS System fo...
Library Number: APNT134841716 | Format: PDF | File Size: 1859.13 kB | Year: 2015 | Content Type: Application Notes

In this study, we demonstrate the benefits of combining RapiFluor-MS with an integrated UPLC-FLR/QTof MS system for detailed characterization of the minor glycoforms from the human IgG and mouse IgG1 samples.

Metabolomics & Lipidomics Application Notebook
Library Number: APNT134841740 | Format: PDF | File Size: 12074.22 kB | Year: 2015 | Content Type: Application Notes

In this notebook, we cover applications with Waters solutions for metabolomics and lipidomics, including untargeted and targeted analyses, structural elucidation, MS imaging, and ambient ionization-MS applications.

Lipid Visualization and Identification Through Collisional Cross Section Aided Correlation of MS Ima...
Library Number: APNT134841560 | Format: PDF | File Size: 2411.75 kB | Year: 2015 | Content Type: Application Notes

This technology brief demonstrates how collisional cross section (CCS) areas can be utilized to correlate MS imaging data with ex situ MS data followed by MS-MS identification.

Quantification of Polysorbate Using the ACQUITY UPLC H-Class Bio System with ELS Detection
Library Number: APNT134841024 | Format: PDF | File Size: 1473.08 kB | Year: 2015 | Content Type: Application Notes

In this work, we present the use of the ACQUITY UPLC H-Class Bio System with a Column Manager and an evaporative light scattering detector (ELSD) for determining the concentration of polysorbate in formulated samples.

Evaluating 2D-RP/RP Fractionation Capabilities of the ACQUITY UPLC M-Class System with 300-μm I.D. C...
Library Number: APNT134840130 | Format: PDF | File Size: 1913.07 kB | Year: 2015 | Content Type: Application Notes

This application note demonstrates the chromatographic performance of the ACQUITY UPLC M-Class System with 2D Technology for the five-step fractionation of two peptide mixtures: MIX-1 MassPREP Digestion Standard and ENL Digestion Standard.

ACQUITY UPC2 Food Application Notebook
Library Number: APNT134840282 | Format: PDF | File Size: 7356.04 kB | Year: 2015 | Content Type: Application Notes

UPC2 enables food scientists to separate, detect, and quantify a broad range of compounds, including fat-soluble vitamins, lipids and fatty acids, structural analogs and isomers, in complex matrices with unequaled speed and confidence.

Methodology for the Identification of Pesticide Metabolites in Complex Matrices Using UPLC-ToF/MS(E)...
Library Number: APNT134840314 | Format: PDF | File Size: 5215.61 kB | Year: 2015 | Content Type: Application Notes

In this application note, we describe a comprehensive approach to metabolite identification in complex matrices using UPLC and high resolution mass spectrometry in conjunction with the UNIFI Scientific Information System

Increasing Sensitivity for Tof-MS Detection of Polychlorinated Biphenyls (PCBs) Using Tof MRM
Library Number: APNT134840001 | Format: PDF | File Size: 1143.85 kB | Year: 2015 | Content Type: Application Notes

In this technology brief, learn how Tof MRM enhances the analysis of PCBs in whale blubber, affording lower limits of detection while maintaining the ability to collect information rich accurate mass full scan data.

New Capabilities for Monitoring Released N-Glycans through the Combined Use of RapiFluor-MS Labeling...
Library Number: APNT134839409 | Format: PDF | File Size: 1483.12 kB | Year: 2015 | Content Type: Application Notes

In this application note, we present the combined use of Rapi Fluor-MS labeling reagent, ACQUITY UPLC H-Class Bio System, and serial fluorescence / ACQUITY QDa Mass Detector for the monitoring of released N-glycan profiles from IgGs. Overall, this new wor...

Transferring Rapi Fluor-MS Labeled N-Glycan HILIC Separations Between UPLC and HPLC
Library Number: APNT134839414 | Format: PDF | File Size: 1372.12 kB | Year: 2015 | Content Type: Application Notes

In the following work, we demonstrate that Glycan BEH Amide Columns packed with 1.7 μm and 2.5 μm particle sizes afford scalability between RapiFluor-MS labeled glycan separations performed under UPLC and HPLC-compatible conditions.

Isolating Trace Impurities for Structural Elucidation in a Commercial Fungicide Formulation Using Pr...
Library Number: APNT134838999 | Format: PDF | File Size: 2792.72 kB | Year: 2015 | Content Type: Application Notes

In this application note, we describe how a workflow to achieve the full structural elucidation of trace impurities can be implemented using preparative supercritical fluid chromatography to isolate trace impurities.

Vial Cap Septum Resealibility after Puncture by LC Sample Manager Needles
Library Number: APNT134838601 | Format: PDF | File Size: 1504.94 kB | Year: 2015 | Content Type: Application Notes

This application note will examine the effect of sample vial cap type on analyte concentration once the cap septum is punctured.

The Regulatory Analysis of Primary Aromatic Amines in Cosmetics and Personal Care Products
Library Number: APNT134838764 | Format: PDF | File Size: 1419.36 kB | Year: 2015 | Content Type: Application Notes

This application note describes an accurate, fast, and robust alternative method for the rapid analysis of PAAs in cosmetic and personal care products, using the ACQUITY UPLC H-Class System coupled with the ACQUITY QDa Detector, and controlled by Empower ...

Multi-Residue Pesticide Analysis in Tea: Optimized Cleanup After QuEChERS Extraction for UPLC-MS/MS ...
Library Number: APNT134775094 | Format: PDF | File Size: 1711.88 kB | Year: 2015 | Content Type: Application Notes

This application note presents QuEChERS extraction and SPE-based cleanup strategies for multi-residue pesticide analysis of dried teas.

Characterization of Biotherapeutics: ACQUITY UPLC H-Class Bio with 2D Part 1 of 3: On-line Desalting...
Library Number: APNT134836823 | Format: PDF | File Size: 2446.9 kB | Year: 2015 | Content Type: Application Notes

In this application note we demonstrate the characterization of biotherapeutics with the ACQUITY UPLC H-Class Bio System with 2D Technology.

Characterization of Biotherapeutics: ACQUITY UPLC H-Class Bio with 2D Part 2 of 3: Rendering a Viabl...
Library Number: APNT134836856 | Format: PDF | File Size: 2723.36 kB | Year: 2015 | Content Type: Application Notes

This application note demonstrates that the ACQUITY UPLC H-Class Bio System with 2D Technology is a viable option for interfacing IEX to electrospray ionization (ESI)-MS.

Characterization of Biotherapeutics: ACQUITY UPLC H-Class Bio with 2D Part 3 of 3: On-line Enrichmen...
Library Number: APNT134836863 | Format: PDF | File Size: 2845.94 kB | Year: 2015 | Content Type: Application Notes

The objective of this application note is to demonstrate that the ACQUITY UPLC H-Class Bio System with 2D Technology is suited for fractionation and enrichment of low abundant species in biological samples.

Non-Targeted Screening Analysis of Packaging Extracts Using the UNIFI Scientific Information System
Library Number: APNT134836651 | Format: PDF | File Size: 2207.96 kB | Year: 2015 | Content Type: Application Notes

This application note describes an LC-MS and Informatics workflow, which employs high-resolution mass spectrometry, that can be adopted for cosmetics, food, and pharmaceutical packaging extractable applications.

Exploiting RapiFluor-MS Labeling to Monitor Diverse N-Glycan Structures via Fluorescence and Mass De...
Library Number: APNT134836333 | Format: PDF | File Size: 1395.8 kB | Year: 2015 | Content Type: Application Notes

In this application note we present the utility of RapiFluor-MS coupled with UPLC-FLR-MS for monitoring labeled glycans ranging across a range of properties, masses, and relative abundance.

Benefits of Using Mass Detection for Assessing Quality and Purity of Cetrimonium Bromide Pharmaceuti...
Library Number: APNT134836200 | Format: PDF | File Size: 1713.63 kB | Year: 2015 | Content Type: Application Notes

In this application note, we present a robust and quick UPLC method for analysis of cetrimonium bromide raw material.

Targeted Lipidomics of Oxylipins (Oxygenated Fatty Acids)
Library Number: APNT134732486 | Format: PDF | File Size: 2178.83 kB | Year: 2015 | Content Type: Application Notes

An SPE UPLC/MRM MS assay is presented for the routine high-throughput detection of more than 100 oxylipins and free fatty acids in plasma.

Routine MS Detection for USP Chromatographic Methods
Library Number: APNT134834272 | Format: PDF | File Size: 2039.87 kB | Year: 2015 | Content Type: Application Notes

This application note presents a systematic protocol for using MS detection directly with unmodified USP methods. The USP assay for Irbesartan is used as an example to illustrate the peak transfer protocol.

Multiple, Sequential DESI Images from a Single Tissue Section at Different Spatial Resolution
Library Number: APNT134833822 | Format: PDF | File Size: 1537.84 kB | Year: 2015 | Content Type: Application Notes

This technology brief demonstrates that desorption electrospray ionization (DESI) imaging can be utilized as a non-destructive imaging technique, which allows multiple analyses in rapid succession on the same tissue section at different spatial resolution...

Metabolic Phenotyping Using Atmospheric Pressure Gas Chromatography-MS
Library Number: APNT134832772 | Format: PDF | File Size: 3853.34 kB | Year: 2015 | Content Type: Application Notes

This application note reports an APGC-TOF MSE profiling approach and its application to metabolic fingerprinting of Arabidopsis.