Literature


Description
Successful Transfer of Size-Exclusion Separations between HPLC and UPLC
Library Number: APNT134822077 | Format: PDF | File Size: 1697.49 kB | Year: 2014 | Content Type: Application Notes

This application note will highlight the considerations that must be made when transferring an SEC method between UPLC and HPLC columns and systems.

Analysis of Artificial Food Dyes Using UltraPerformance Liquid Chromatography and an Extended Wavele...
Library Number: APNT134821857 | Format: PDF | File Size: 1721.35 kB | Year: 2014 | Content Type: Application Notes

This application note presents a fast, efficient method for the analysis of food dyes in a variety of foods.

Analysis of Glyphosate, Glufosinate, and AMPA in Tap and Surface Water Using Open-Architecture UPLC ...
Library Number: APNT134821560 | Format: PDF | File Size: 1613.96 kB | Year: 2014 | Content Type: Application Notes

In this application, the analysis of glyphosate, glyfosinate, and AMPA in water was performed using three automated sequences for the derivatization and separation.

The Use of HDMS Compare Software for Comparison of Complex Polymeric Materials
Library Number: APNT134821222 | Format: PDF | File Size: 1138.06 kB | Year: 2014 | Content Type: Application Notes

In this study HDMS® Compare Software together with ion mobility mass spectrometry (IMS) has been evaluated as a comparative analytical approach to highlight and detect differences in batches of D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) pro...

Quantitative Analysis of Astaxanthin in Dietary Supplements by UltraPerformance Convergence Chromato...
Library Number: APNT134820709 | Format: PDF | File Size: 1642.1 kB | Year: 2014 | Content Type: Application Notes

Quantitation of Astaxanthin was determined in this application note.

Improving the Productivity in Isolating a Naturally Occurring Bioactive Compound Using Supercritical...
Library Number: APNT134820723 | Format: PDF | File Size: 1665.45 kB | Year: 2014 | Content Type: Application Notes

In this application note, we have demonstrated employing SFE and prep SFC to holistically improve the productivity in isolating a low-polarity, bioactive compound from a complex natural product extract.

Analysis of Pharmaceuticals and Pesticides in Bottled, Tap, and Surface Water Using ACQUITY UPLC Sys...
Library Number: APNT134820733 | Format: PDF | File Size: 2654.69 kB | Year: 2014 | Content Type: Application Notes

The application targeted the analysis of PPCP’s and pesticides in bottled, tap, and surface water.

Analysis of 2- and 4-Methylimidazole in Beverages using Alliance HPLC with Mass Detection
Library Number: APNT134820627 | Format: PDF | File Size: 1773.03 kB | Year: 2014 | Content Type: Application Notes

In this application note we show a rapid analysis (<8.5 min) for 4-MEI and its isomer, 2-MEI using HPLC separation on a Waters CORTECS Column with mass detection.

Advanced HPLC Size-Exclusion Chromatography for the Analysis of Macromolecular Proteins Using 3.5 μm...
Library Number: APNT134820571 | Format: PDF | File Size: 1814.57 kB | Year: 2014 | Content Type: Application Notes

This application note highlights the performance characteristics of both the 200Å and 450Å pore-size versions of columns, designed for the separation of macromolecular proteins, with respect to resolution, column-to-column reproducibility, and column stab...

A Validated Assay for the Quantification of Amino Acids in Mammalian Urine
Library Number: APNT134820198 | Format: PDF | File Size: 1657.54 kB | Year: 2014 | Content Type: Application Notes

In this application note a robust, reliable method for the absolute quantification of twenty amino acids and the relative quantification of a further eighteen amino acids in human urine has been developed and evaluated.

Increasing Sensitivity and Minimizing Sample Volume for the Quantification of Therapeutic and Endoge...
Library Number: APNT134817596 | Format: PDF | File Size: 3250.22 kB | Year: 2014 | Content Type: Application Notes

This application investigates the improved sensitivity and decreased sample volume requirements for the therapeutic and endogenous cyclic peptides: desmopressin, vasopressin and octreotide.

Enantiomeric Separation of Warfarin and Propranolol and their Associated Hydroxylated Metabolites us...
Library Number: APNT134819888 | Format: PDF | File Size: 785.57 kB | Year: 2014 | Content Type: Application Notes

In this technology brief, we demonstrate the ease of enantiomeric separation and detection of warfarin, and three hydroxylated metabolites (Figure 1), as well as propranolol and three hydroxylated metabolites utilizing UPC2-MS/MS.

Exploring the Benefits and Potential of iKey Microfluidic Chromatography and Time-of-Flight Mass Spe...
Library Number: APNT134819767 | Format: PDF | File Size: 3297.6 kB | Year: 2014 | Content Type: Application Notes

Enhanced ionization/transmission efficiency provides higher sensitivity to detect pesticides residues in complex food commodities at regulatory limits.

Automating the Determination of Drug-to-Antibody Ratio (DAR) of Antibody Drug Conjugates (ADCs) Base...
Library Number: APNT134819475 | Format: PDF | File Size: 2992.69 kB | Year: 2014 | Content Type: Application Notes

We demonstrate the ability to automatically determine DAR values corresponding to the drug load distribution of cysteine-conjugated ADCs using Empower 3 Chromatography Data Software.

Enantiomeric and diastereomeric separations of fragrance and essential oil components using the ACQU...
Library Number: APNT134819179 | Format: PDF | File Size: 1381.22 kB | Year: 2014 | Content Type: Application Notes

In this application note, we have demonstrated the successful chiral separations of fragrance compounds on ACQUITY UPC2 Trefoil AMY1 and CEL1 Columns using an ACQUITY UPC2 System.

Analyzing Pharmaceutical Degradation Products Using the New ACQUITY UPC2 Torus Columns
Library Number: APNT134818380 | Format: PDF | File Size: 980.95 kB | Year: 2014 | Content Type: Application Notes

This technology brief demonstrates the performance of the new ACQUITY UPC2 Torus™ 2-PIC Column for analyzing low level pharmaceutical degradation products.

Measurement of Urinary Serotonin for Clinical Research, Using Mixed-Mode SPE and a High-Strength Sil...
Library Number: APNT134818422 | Format: PDF | File Size: 1364.78 kB | Year: 2014 | Content Type: Application Notes

This application note details the use of mixed-mode ion exchange solid phase extraction (SPE) to selectively isolate serotonin from urine, followed by chromatographic separation on a high-strength silica (HSS) PFP column.

Streamlining Analysis of Impurities in the Pharmaceutical Products using Empower 3 ICH Impurity Proc...
Library Number: APNT134818261 | Format: PDF | File Size: 1641.43 kB | Year: 2014 | Content Type: Application Notes

This technology brief demonstrates the use of Empower 3 ICH Impurity Processing for streamlining the analysis of impurities of Ziprasidone HCl by defining allowable threshold limits and quickly identifying results above the limits.

Rapid Screening of 36 Synthetic Dyes using the ACQUITY UPLC H-Class System with the ACQUITY QDa Dete...
Library Number: APNT134818329 | Format: PDF | File Size: 594.27 kB | Year: 2014 | Content Type: Application Notes

In this technology brief, we describe a method to selectively analyze 36 disperse, basic, acid, solvent and direct synthetic dyes to below EU legislative limits.

Analysis of Beta-Blockers Using UPLC with Accurate-Mass Screening
Library Number: APNT134817523 | Format: PDF | File Size: 2117.65 kB | Year: 2014 | Content Type: Application Notes

This application note describes the analysis of Beta-blockers in Forensic toxicology applications.

Advantages of CORTECS C18 2.7 μm and XBridge BEH Phenyl XP 2.5 μm Columns for the Analysis of a Comp...
Library Number: APNT134817588 | Format: PDF | File Size: 1757.77 kB | Year: 2014 | Content Type: Application Notes

This application note highlights the analysis of a comprehensive panel of opiates, benzodiazepines, and other drugs of abuse.

Polar Basic Drugs in Environmental Samples; Improved Analysis Using a New High Efficiency UPLC Colum...
Library Number: APNT134817340 | Format: PDF | File Size: 1425.63 kB | Year: 2014 | Content Type: Application Notes

The purpose of this application note is to demonstrate the CORTECS UPLC HILIC Column for determination of highly polar basic compounds.

A Simplified Solid Phase Extraction (SPE) Protocol for Bioanalysis Using Oasis HLB
Library Number: APNT134817360 | Format: PDF | File Size: 1808.33 kB | Year: 2014 | Content Type: Application Notes

In this application note, we introduce a simple, 3-step SPE protocol using Oasis HLB in μElution Plates and 1 cc cartridges.

Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve F...
Library Number: APNT134816984 | Format: PDF | File Size: 2335.92 kB | Year: 2014 | Content Type: Application Notes

In this study, which employs a SYNAPT G2-S instrument, we determine and compare the quantification attributes of detection limit (LOD), quantification limit (LOQ), and linear dynamic range for three data-acquisition modes: full-scan MS, MS,E and Tof-MRM.

Enantiomeric Separation of the Active Metabolite of Risperidone and its Application for Monitoring M...
Library Number: APNT134817007 | Format: PDF | File Size: 2372.57 kB | Year: 2014 | Content Type: Application Notes

In this technology brief, we demonstrate the enantiomeric separation and detection of 9-hydroxyrisperidone.