High-Resolution Separation of Intact Antibodies Using BioResolve RP mAb Polyphenyl Columns

Library Number:
APNT134966185
Part Number:
720006203EN
Author(s):
Ximo Zhang, Robert Birdsall, and Ying Qing Yu
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 

This application note provides a high-resolution, reversed-phase based separation method for intact antibodies that take advantage of the purposefully-designed capabilities of the BioResolve RP mAb Polyphenyl Column. Using optimized, silica-based, solid-core particles that are surrounded by a porous layer containing 450Å pores with novel polyphenyl bonded phase, the BioResolve RP mAb Polyphenyl Column increases the efficiency and selectivity of intact antibody separations. This results in improvements in resolution, peak width, peak shape, and carryover compared to current leading porous and non-porous column technologies.


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