Here we address the challenges faced trying to routinely measure 17β-Estradiol (E2) and Estrone (E1) at levels of 3.7pmol/L (1pg/mL) by LC-MS/MS for clinical research applications. The quest to reach these low levels led us to investigate a range of sample preparation and liquid chromatography methods. Samples were analyzed using an ACQUITY® UPLC® I-Class-FTN and Xevo® TQ-XS mass spectrometer for optimum analytical sensitivity. Limits of quantification will be presented to show ‘how low you could go’ with this routine LC-MS/MS workflow without the need for derivatization.
For Research Use Only, Not for use in diagnostic procedures.