Targeted liquid chromatography - mass spectrometry (LC-MS) assays, using unit mass resolution, tandem quadrupole mass spectrometers, and standard analytical flow UPLC, are increasingly being used in the discovery omics arena. This approach offers an alternative to the untargeted, high-resolution, approach traditionally applied in these types of studies, using time of flight instruments and micro/nano flow LC. These methods offer increased simplicity and throughput, while still offering the level of sensitivity and specificity required.
Here, this methodology is applied to metabolomic, lipidomic and proteomic preparations of a set of human plasma samples, using the same LC-MS platform. The ability to run these 3 different analysis types on the same LC-MS platform facilitates the consecutive analysis of multiple sample sets from different sample preparations with virtually no down time.