Accurate Quantification of intact Insulin-like Growth Factor I (IGF-I) using High Resolution Mass Spectrometry (HRMS) for Clinical Research

Library Number:
APNT134965293
Part Number:
720006167EN
Author(s):
Nikunj Tanna, Mary Lame, Ian Edwards, and Mark Wrona
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
Protein Quantification
 

This technology brief demonstrates analytically sensitive, accurate, and robust quantification of intact IGF-I in biological matrix using a simple sample preparation workflow, an analytical LC and HRMS.

HRMS platforms, with their ability to provide qualitative and quantitative information offer a flexible alternative to TQ systems, especially for direct quantification of intact proteins. With improvements in hardware and software, HRMS platforms can now achieve the performance characteristics required for bioanalytical quantification, as highlighted by the analytically sensitive (10 ng/mL) and robust (CV’s <10%) quantification of intact IGF-I for clinical research.


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