A UPLC-MS/MS based multiresidue, multiclass method was developed and validated for the simultaneous confirmation and quantification of 24 analytes of quinolones including fluoroquniolones, sulfonamides and tetracyclines and its epimers in crustaceans (shrimp tissue) following the European Union’s (EU) performance criteria (CD 2002/657/EC).
Samples were extracted using a simple method by acidified acetonitrile (0.5 mL of 0.1% formic acid in 7 mL of Acetonitrile) with a clean-up using n-hexane and methanol, followed by Ultra Performance Liquid Chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) within 8 minutes using positive mode. The method validation as per CD 2002/657/EC and demonstration through proficiency testing provided evidence that the method is suitable to be applied in detection and quantification of 24 antibiotic residues in routine analysis of fish and fishery products.
Hosted by Food Safety Magazine
Participants will learn:
Anoop A Krishnan, Ph.D.
Assistant Director (Technical),
Export Inspection Agency- Kolkata (Laboratory), India
Dr. Anoop A Krishnan (Ph.D in Biosciences, Mangalore University) is currently the Assistant Director and Lab In Charge at the Export Inspection Agency Kolkata (Laboratory) in New Delhi. He has worked in food safety, chemical residues & contaminant analysis for nearly 15 years, and with the application of mass spectrometry in food safety, residue and contaminant analysis for over 10 years. In his current role at Export Inspection Agency Kolkata, he is responsible for the development of new method in residues and contaminant analysis.