Advancing Host Cell Protein Analyses through the Combined Use of Microscale 2D RP/RP with CSH C18 and Ion Mobility Enabled MS Detection

Library Number:
APNT134800694
Part Number:
720005076EN
Author(s):
Matthew A. Lauber, Catalin E. Doneanu, Stephan M. Koza, Weibin Chen, and Kenneth J. Fountain
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
SYNAPT G2-S MS
 
 
 
 

Here, recent advances in microscale peptide chromatography are applied to HCP analysis. The novel charge-surface-modified C18 material, known as CSH C18, which can produce high peptide peak capacities even at high mass loads, is used in the format of a 15K psi capable 300 μm I.D. column along with an ACQUITY UPLC M-Class based 2D-RP/RP system and SYNAPT HDMSE detection to provide a robust analytical workflow for the identification of low concentrations of HCP impurities at single digit PPM levels (parts per million, or ng/mg).


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