Benefits of Mass Detection for Routine LC Analysis of Tobacco Products

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Naren Meruva, Yinchuan Yang, Rainer Rozenich
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There are several tobacco industry methods that rely on HPLC separation and optical detection (UV, Fluorescence and ELSD) for identification of target compounds such as carbonyls, nicotine, related alkaloids, sugars and artificial sweeteners. In this application we demonstrate the benefits of QDa mass detector for method development, transfer and routine analysis of tobacco products in combination with UPLC and optical detection workflow.   

Peak traceability can be a challenge during method development and routine testing using optical detectors. E-liquid formulations were analyzed under different mobile phase pH conditions and using different UPLC columns to show the benefits of MS detection during method development. In a second example, we show the benefits of using UPLC to drastically reduce the analysis time for determination of carbonyl compounds but also demonstrate how the addition of a mass detector helps to confirm trace analytes and avoid interference from co-eluting compounds.

Finally the benefits of using dual detection (UV and MS) for simultaneous analysis of nicotine and related impurities demonstrate how to measure the major and minor components of a e-liquid formulation in a single analysis. These experiments demonstrate that the use of mass detection greatly reduces the method development and transfer time, identifies interferences from co-eluting compounds, reduces troubleshooting time for out of specification results, which will in turn improve overall lab productivity and analytical data quality.

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