LC-MS/MS Quantification of Intact Insulin-Like Growth Factor I (IGF-I) in Serum

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Nikunj Tanna, Mary Lame, Mark Wrona, and Erin Chambers
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Protein Quantification

The novel work described here provides a simple and cost effective sample preparation workflow and LC-MS/MS method, which uses mixed-mode solid-phase extraction (SPE) in a 96-well format and analytical scale LC with a sub-2-um column coupled to a high-performance tandem quadrupole TQ-XS, for the accurate and robust quantification of intact IGF-I, achieving LLOQ’s of 5 ng/mL.

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