A Comprehensive Comparison of Solid Phase Extraction (SPE) vs. Solid Liquid Extraction (SLE) vs. Liquid Liquid Extraction (LLE) Sample Prep Techniques in Bioanalysis and Forensic Toxicology Analyses

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Jonathan P. Danaceau, Kim Haynes, and Erin E. Chambers
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In this application, a comprehensive comparison of sample preparation techniques including SPE, SLE, and LLE was conducted in plasma and urine, using a wide variety of compounds found in bioanalysis and forensic toxicology.

Oasis PRiME HLB also demonstrated superior recoveries and matrix effects for a variety of tested drugs without any additional method development. SLE and LLE required additional method development or multiple extraction protocols to achieve recoveries that were comparable to Oasis PRiME HLB for all of the tested analytes. The unique nature of Oasis PRiME HLB enabled the elimination of conditioning and equilibration steps, simplifying the extraction procedure and speeding up the sample preparation workflow. The μElution format enabled the direct injection of extracts without evaporation or reconstitution.

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