Analysis of Fat-Soluble Vitamins Using UPLC with PDA and the SQ Detector
A 5-minute method utilizing UPLC has been developed to detect fat-soluble vitamins. Current published methods typically require at least 15 minutes to perform the same analysis. While identification of these vitamins is possible using a PDA, samples are often complex, requiring additional techniques to detect all components of interest. The PDA and SQ detectors are complementary techniques that allow not only for the identification of vitamins but also for components that may not be amenable to PDA detection alone.