Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and the ACQUITY QDa Detector

Library Number:
APNT134784742
Part Number:
720004960en
Author(s):
Mark E. Benvenuti, Dimple Shah, and Jennifer A. Burgess
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
 

Rapid, reliable, and cost-effective methods are required by food manufacturers and ingredient suppliers in order to verify product consistency and ensure that label claims are met. This can be a challenging task with the combination of complex matrices and low fortification levels of some vitamins. In addition, many of the methods currently employed stipulate that the vitamins are either analyzed separately, or in small groups. Established techniques include microbiological assays, colorimetric and fluorimetric analysis, titrimetric procedures and HPLC methodologies. LC-MS offers the opportunity to consolidate methods along with the ability to improve detector selectivity and reduce limits of quantification. In order to offer laboratories the opportunity to capture the benefits of mass detection without the challenges associated with the adoption of mass spectrometers, recent advances in technology have focused on improving instrument usability and robustness. These motivations have resulted in the introduction of the ACQUITY QDa Detector. In this application note, 12 water soluble vitamins (WSVs) were analyzed in dietary supplements and beverage samples using the ACQUITY UPLC H-Class System with t he ACQUITY QDa Detector.


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