Development of a Metabolomic Assay for the Analysis of Polar Metabolites Using HILIC UPLC/QTof MS

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Giuseppe Paglia, James Langridge; and Giuseppe Astarita
Waters Corporation; and Center for Systems Biology, University of Iceland, Iceland
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The combination of UPLC-based hydrophilic interaction liquid chromatography (HILIC) and a hybrid quadrupole time-of-flight (Q-Tof) mass spectrometer allows the comprehensive analysis of small polar metabolites including sugars, phosphorylated compounds, purines and pyrimidines, nucleotides, nucleosides, acylcarnitines, organic acids, hydrophilic vitamins, and amino acids. Retention times and accurate masses of metabolites involved in key metabolic pathways were annotated for routine high-throughput screening in both untargeted and targeted metabolomics analyses.

A HILIC-UPLC/MS strategy for the routine high-throughput screening of polar metabolites in biological samples is presented in this work. Such a strategy could be used for both targeted and untargeted metabolomics.

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