Vitamins are minor constituents that have to be introduced, via our food, in small quantities because they are not synthesized by the human body. The vitamin composition of infant formula is critical for correct infant development, particularly if the mother is unable to breast-feed and formula is the primary source of nutrition. Official analytical methods for the determination of water-soluble vitamins are based on procedures, mainly microbiological assays, which have been established for decades Each vitamin is analyzed separately in order to apply extraction conditions, which permit the determination of its total content in a food. Vitamin analysis in food is generally a time-consuming process.
In this application note, we describe a rapid, five-minute UPLC/MS/MS method using positive ESI ionization for the simultaneous analysis of 12 water-soluble vitamin compounds in infant formula.