Rapid and Simultaneous Analysis of Urinary Catecholamines and Metanephrines Using Mixed-Mode SPE and Hydrophilic Interaction Chromatography (HILIC) for Clinical Research

Referencia en la biblioteca:
APNT134807288
Referencia:
720005093EN
Autor(es):
Jonathan P. Danaceau, Erin E. Chambers, and Kenneth J. Fountain
Fuente:
Waters
Tipo de contenido:
Aplicaciones
Subtipo de contenido:
Aplicaciones
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The extraction and analysis of urinary catecholamines and metanephrines using the Oasis mixed-mode weak cation exchange (WCX) plates and an ACQUITY UPLC BEH Amide Column in HILIC mode is detailed. Extraction using the Oasis WCX Plate resulted in low matrix effects and consistent recoveries for all compounds that translated into excellent analytical precision. HILIC separation resulted in reduced matrix effects for NE and EP and improved resolution between EP and NMT, compared to optimized reversed-phase separations. Quantitative results were excellent, with linear responses from 0.5-500.0 ng/mL and excellent accuracy and analytical precision.


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