Exploiting the Orthogonality of the ACQUITY UPC2 System to Develop an Impurities Method for Ondansetron

Library Number:
APNT134903247
Part Number:
720005732EN
Author(s):
Jennifer Simeone, Paula Hong and Patricia R. McConville
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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This application note describes retention of small polar compounds using UPC2 and simplified method development using ACQUITY QDa.

Supercritical fluid chromatography (SFC) is known to be orthogonal to RPLC, and uses reagents which are suitable for MS detection. In this study, eight impurities of ondansetron, including two polar impurities imidazole and 2-methyl imidazole, were easily retained and separated from the API using SFC coupled with UV and MS detection.


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