This application note illustrates a streamlined workflow using UPLC with ion mobility time-of-flight MSE (HDMSE) in conjunction with SimLipid Software for the high throughput and automated identification of lipids in complex biological matrices. UPLC and HDMSE provide multiple degrees of orthogonal separation and unprecedented peak capacity, and, for lipidomic analyses, produce a large amount of data. SimLipid Software facilitates this data processing to increase confidence in interpreting lipidomics datasets and identifing lipids.
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