Waters would like to invite you to join us for our lunch symposium at 7th Asia Oceania Mass Spectrometry Conference (AOMSC).
The AOMSC has been a successful and useful conference to showcase advancement in mass spectrometric developments and applications, and also serve as a forum to foster closer cooperation and collaboration between mass spectrometry practitioners.
We are particularly proud to be able to present two distinguished speakers who are both pioneers and innovators in their field of research. Participants will have the opportunities to gain insights to current trends in MS DESI imaging and LC-MS/MS quantitation.
Topic 1: DESI imaging of vulnerable plaques of the artery
Prof Mitsutoshi Setou
DESI imaging revealed the vulnerable plaque is the target of M2 macrophage where dietary eicosapentanoic acid is converted to anti-inflammatory 12-hydroxy-eicosapentanoic acid.
In this study, ApoE knockout mice (a model of atherosclerosis) fed a Western diet only, EPA+Western diet, and docosahexaenoic acid (DHA)+Western diet for 3 weeks were used as the control, EPA, and DHA groups, respectively. DESI based Imaging mass spectrometry (MS) was performed to analyze the fat distribution of aortic arch and aortic valve plaques. Histological analysis and liquid chromatography/tandem MS (LC-MS/MS) were performed to analyze the fat composition of the aortic arch, in order to clarify the effect of EPA administration in changing the local fat distribution and macrophage distribution of vulnerable plaque.
Topic 2: LC-MS/MS Quantitation of 8-iso-prostaglandin F2α, a Marker of Oxidative Stress, in Human Plasma Samples
Prof Chester Drum
F2-isoprostanes (F2-isoP) are prostaglandin-like compounds formed in vivo via a non-enzymatic mechanism involving the free-radical catalyzed peroxidation of arachidonic acid, and are associated with oxidative stress, oxidative damage and antioxidant deficiency. They also play a vital role as vasoconstrictors within the vasculature of the heart, brain, lung, intestine, and kidneys. Elevated F2-isoprostanes levels in blood or urine have been observed patients with neurodegenerative diseases, liver diseases, asthma, pulmonary disease, diabetes and acute myocardial infarction. The most common, reliable, specific and non-invasive "gold standard" test for quantifying lipid peroxidation/oxidative stress in vivo is the 8-iso-prostaglandin F2α (8-IsoP). Current GC-MS method is sensitive, but tedious and time-consuming due to the requirement of a derivatization step prior to separation. Cross-linking reactions can be observed in commercially available ELISA, and each assay kit can only measure just one isomer of F2-isoprostane. We report, for the first time, that we have developed a highly sensitive and specific method for the separation and quantification of 8-IsoP, using just 20mL of extracted human plasma with an assay run-time under 7min.