XBridge Columns, utilizing BEH particle technology, are built to withstand high pH chromatography, high temperatures chromatography, and other extreme operating conditions, including volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis. These columns are available in a broad range of sorbent particles and sizes. XBridge XP Columns (2.5 µm) for rapid analytical UHPLC separations, XBridge BEH Columns (3.5 and 5 µm) for reliable analytical HPLC method development and XBridge OBD Prep Columns (5 and 10 µm) for purification chromatography. XBridge BEH Columns allow separation scientists to easily scale from analytical UHPLC/HPLC to preparative HPLC. XBridge BEH sorbents provide it all, from VanGuard pre-columns and column guards to extend column lifetime, to method validation kits, you'll expect:
XBridge Columns are offered in 14 phases. General use phases can be used across a wide variety of applications. XBridge also offers phases are designed for analysts working with proteins, peptides, oligonucleotides, glycans, and size exclusion chromatography.
|C18||Peptide BEH C18 130 Å||Protein BEH C4 300 Å||BEH SEC 125 Å|
|C8||Peptide BEH C18 300 Å||BEH SEC 200 Å|
|Shield RP18||Oligo BEH C18||BEH SEC 450 Å|
|Phenyl||Glycan BEH Amide|
Mechanical Strength and Chemical Stability
Ethylene Bridged Hybrid (BEH) Technology synthesis creates particles that ensure extreme column performance and longer column lifetimes under all chromatographic conditions. Hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control surface silanol activity for better reproducibility, peak shape and retention time. The BEH particle (1.7, 2.5, 3.5, 5, and 10 μm) is prepared from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in a highly stable, pH resistant, and mechanically strong particle allowing seamless transfer from UPLC, UHPLC, HPLC and preparative separations. (See Related tab for more info on Ethylene-Bridged Hybrid (BEH) Technology).
pH Lifetime and Reliability
XBridge BEH HPLC and UHPLC Columns offer mobile phase pH stability that is higher than any other commercially available chromatographic phase. Unlike approaches that claim high pH resistance, the pH stability of XBridge BEH Columns is created by utilizing proprietary surface modifications, is incorporated into the particle synthesis process.
This XBridge BEH C18 HPLC Column was tested under accelerated pH 10 stability test conditions against some of the most popular chromatographic phases that claim extended high pH stability. The XBridge BEH HPLC Column showed very little degradation in chromatographic performance, exceeding the performance of the best silica column by over 1000%.
XBridge Columns are designed to eliminate the compromise of sorbent selection and deliver the flexibility to work under any mobile phase, temperature and pH chromatography conditions. Even the most challenging sample mixtures can be quickly resolved with a sample method development protocol consisting of XBridge HPLC Columns, two mobile phase pH’s and two organic solvents.
All XBridge BEH Columns are designed for stability to preserve separation efficiency and seamless method transfer throughout the available particle sizes (1.7, 2.5, 3.5, 5, and 10 μm). With XBridge BEH Columns, you have the column configurations and particle sizes required to meet the most demanding chromatographic challenges. Whether you require robust HPLC methods, seamless UPLC/UHPLC transferability, or preparative scaling for product isolation.
Preparative Separation Performance
Waters innovative Optimum Bed Density (OBD) Preparative Column design ensures direct scalability that is independent of particle size. The combination of the revolutionary OBD format with the BEH base particle delivers the most stable, efficient, and reproducible preparative LC columns available. XBridge BEH OBD Preparative Column users benefit from high pH and temperature stability for increased mass loading for basic compounds.
XBridge BEH HPLC and UHPLC Columns are available for application specific analysis. These are dedicated high-performance sorbents for isolation, analysis, and characterization of biomolecules. Innovative XBridge BEH Columns support UHPL and HPLC for LC-MS analyses as well as lab scale purification of oligonucleotides, amino acids, peptides, proteins and associated glycans.
Protect your Column without Compromise
Don’t take chances… Complex sample matrices encountered in pharmaceutical, natural product, environmental, and industrial chemical analysis can prematurely shorten analytical column lifetime and degrade chromatographic performance. Using guard cartridges and pre-columns like VanGuard Column Protection products improve analytical column performance by removing particulate and chemical contamination that may be present in the mobile-phase stream—without compromising chromatographic performance.
Improve analytical column performance by removing particulate and chemical contamination.
VanGuard Column Protection