By upgrading your SYNAPTTM MS series instrument to full HDMSTM capability you can take advantage of high efficiency ion mobility separations (IMS) to extend your research to a new dimension. The HDMS upgrade will enable you to differentiate samples based on size, shape, and charge, as well as mass. This provides a range of new or improved analytical benefits such as conformational analysis, the ability to separate different compound classes, remove background and interferences, or carry out comprehensive structural characterization.
Ion mobility/HDMS mode expands your capabilities beyond those of your present SYNAPT MS series instrument by incorporating:
MALDI source users can also benefit significantly from the HDMS upgrade particularly with imaging applications using the Waters High Definition Imaging (HDITM) MALDI solution. HDI MALDI helps generate images without interference from background ions of similar mass, leading to more precise localization of the drug or metabolite of interest.
Triwave™ Technology is the enabling system behind the SYNAPT™ MS series HDMS™ field upgrade. It uses three T-Wave™* ion guides that trap ions, separate them by their ion mobility and transfer them to the orthogonal acceleration time-of-flight analyzer for high-resolution mass analysis. The trap and transfer regions can also be used for fragmentation analyses.
The Triwave Technology’s innovative configuration overcomes the major performance drawbacks of conventional ion mobility spectrometry (IMS) by ensuring that IMS does not compromise the base sensitivity of the instrument.
*The Traveling wave device described here is similar to that described by Kirchner in US Patent 5,206,506; 1993.
The combination of MALDI and the ion mobility field upgrade allows MALDI users to isolate analyte ions from multiply charged background ions & matrix ions, enabling the separation of molecular species by structural class. This is particularly important for MALDI imaging applications as ion mobility separation has been shown to separate isobaric interferences from imaging data obtained from highly complex biological samples, so increasing confidence in the resulting image.
This example illustrates how singly charged ions can be separated from multiply charged components using MassLynx™ or DriftScope™ Software, thereby simplifying data interpretation. Further investigation, (shown in the detailed image), reveals the separation of isobaric peptides by drift time. A subsequent database search confirms the presence of isobaric peptides, resulting in increased protein coverage for the experiment.
DriftScope Sofware shows data of different charge states separated by drift time. Detail shows isobaric precursors separated by drift time.
Unlock the full power of the SYNAPT MS series HDMS field upgrade
DriftScopeTM Mobility Environment Software incorporates new technologies to enable comprehensive retrieval of information contained within HDMSTM (ion mobility) datasets while enabling easier data interpretation (for users of all experience levels) through a series of data visualization and data export enhancements:
Powerful and automated peak detection (novel APEX 3D and 4D technology)
Visualization enhancements (e.g. 3D peak profile display, x/y axis switching, peak/raw data overlay, lock-axis function, ability to turn raw data on/off)
Data export functionality (raw data or peak list export and peak copy/paste function)
Enhanced analytical power
DriftScope mobility environment software provides automated peak detection from 2D (Chromatograms and Mass Spectra), or 3D graphical plots. The axes are interchangeable, each pixel representing an ion, and its color, the intensity. The peaks are represented as colored (green) dots and sticks. The data shown is from the Electrospray (infusion-based) IMS-MS analysis of a Polyethylene Glycol (PEG) sample.
Conformation (IMS) Studies
Measuring the mobility (or drift time) of an ion can yield information about its structure, which you can then use to investigate the size and shape of small and large molecules.
Molecular conformation is critical to the integrity of many biological systems, which means that increased knowledge of the shapes of biologically relevant components (such as proteins, peptides, carbohydrates, and nucleotides) can benefit many research areas.
Analysis of the native and reduced forms of the intact protein Hen Egg Lysosyme using Mobility-TOF (HDMS™) mode on the SYNAPT™ HDMS System. The DriftScope™ Software plot shows m/z versus drift time. Upon acidification and reduction of the native form (single drift time distributions), multiple drift time distributions are observed for the two charge-states shown. This indicates a change in the overall size and shape (topology) of the protein has taken place. Average rotational cross-sections can be determined for the individual conformers from these data.
Reduce Sample Complexity
The SYNAPT™ MS series instrument HDMS™ upgrade provides a unique way to increase the information content for a variety of applications. DriftScope™ Software eliminates common interferences from an analysis, providing access to lower-level components:
UPLC®/IMS-TOF sample analysis of a rat bile sample containing the dosing vehicle (PEG), parent drug and its metabolites. The circled regions can be easily removed from the data set using DriftScope Software (e.g. PEG dosage vehicle). This provides a simple, unique route to enhanced MS detection of low-level components.
Analysis of released glycans from IgG using a MALDI SYNAPT HDMS System showing the effect of using ion mobility to remove background ions.
The SYNAPT™ MS series instrument HDMS™ upgrade provides an expanded range of fragmentation protocols for structural characterization studies through use of the trap and transfer areas of the Triwave™ to induce fragmentation.
Time Aligned Parallel (TAP) Fragmentation Triwave Technology allows the combination of high-efficiency ion mobility spectrometry with a dual-stage fragmentation process for detailed structural characterization.
TAP Fragmentation: All first generation product ions are fragmented in the Transfer T-Wave – a Parallel process; the second generation product ions are Time Aligned to original first generation ions.
Equipment and Installation
The SYNAPT™ MS series HDMS™ system upgrade is available for the following instruments:
The upgrade kit includes:
The upgrade requires installation of the above hardware and new system software and firmware to reconfigure the present Triwave gas cell technology to full High Definition MS functionality. DriftScope Mobility Environment software will also be installed. The work is carried out by a certified Waters Field Service Engineer who also provides initial operator training.
On completion of the HDMS upgrade, the total MS instrument including MALDI source where applicable, is warranted against component failure for a further 90 days.