SYNAPT G2-S HDMS

When your progress is limited by missing information or the risk of false positives in your analysis, the combination of high-sensitivity StepWave™ ion optics, the QuanTof™ analyzer and MS^E ‘data-independent’ acquisitions will maximise your chances of success.

These unique innovative technologies deliver the highest, all-inclusive qualitative and quantitative performance attributes, for UPLC^® based analysis of complex mixtures. With all this available through a simple, generic analytical methodology that delivers comprehensive coverage, why look any further?

• Selectivity and Accuracy –Utilize high resolution, exact mass, accurate isotope abundance, maximum dynamic range and speed of acquisition, all together, all of the time, with UPLC separations and QuanTof
• Sensitivity and Linearity –Access exceptional linearity, reproducibility and sensitivity in the most complex matrices with StepWave and QuanTof
• Maximum Coverage – Eliminate the risk of incomplete analysis with UPLC/MS^E, a simple patented method of data acquisition that comprehensively catalogs samples in a single analysis
• Simplify and Extend – For untargeted quantification, leverage the  benefits of oa-Tof and MS^E
  • Quantify and confirm hundreds or thousands components in a single assay
  • Eliminate or reduce time-consuming MS method development
  • Re-interrogate archived datasets to quantify or identify new compounds

SYNAPT^® G2-S High Definition MS™ with Triwave^® technology – every scientist can access unparalleled information content and unique proven discovery capabilities for their toughest analytical challenges, by combining high-efficiency ion mobility separations with high resolution exact mass tandem MS

Unparalleled Analytical Peak Capacity

By combining high-efficiency ion mobility separations with a high resolution time-of-flight analyzer, SYNAPT G2-S provides a higher peak capacity and greater information content than is available with the highest chromatographic or mass resolution alone.

Enhanced conformational and structural characterization

Collision Cross Section (CCS) – Because Shape Matters

Molecular conformation information provides a unique complement to traditional methods of structural determination.

CCS values can be determined easily using High Definition MS, leading to accurate conformational measurements for both small molecules and large protein complexes.

Time Aligned Parallel (TAP) Fragmentation – For More Complete Structural Elucidation

TAP fragmentation enables a more complete investigation of molecular structure than traditional MSⁿ or MS/MS techniques.  Ion mobility separation plays a key enabling role, separating first generation fragment and second generation product ions and aiding the automated assignment of fragments by the software.

SYNAPT^® G2-S serves the broadest range of applications by providing the most extensive range of targeted data acquisition, chromatographic inlet and ion source capabilities.

Exact Mass ETD – When CID Isn’t Enough - When analyzing post-translational modifications and top-down sequencing is all important, Electron Transfer Dissociation (ETD) complements Collision Induced Dissociation (CID). Developed specifically to maximise confidence, flexibility and ease-of-use the optional ETD capability of SYNAPT G2-S provides a uniquely powerful platform for sequencing of biomolecules.

FastDDA - UPLC^®/FastDDA delivers rapid, automated, intelligent exact mass MS/MS capability for full structural characterisation and isobaric labelling strategies in quantitative proteomics experiments

MS^E - UPLC/MS^E and HDMS^E uses a simple, patented method of data acquisition to comprehensively catalogue complex samples in a single analysis.

MS and MS/MS Flexibility - The QuanTof analyzer provides a mass range of up to 100,000 m/z for mass analysis - and a range of quadrupole options allow molecular species up to 32K Da in mass to be selected for MS/MS.

The UPLC Advantage - The ACQUITY Ultra PerformanceLC^®family of products is proven to be the most powerful and flexible chromatographic inlet for mass spectrometry based analysis today.

Ion Source Flexibility

Future-proof your analytical needs of tomorrow with Waters Universal Ion Source Architecture, engineered to take maximum advantage of UPLC, allows the widest range of ionization techniques as well as the very latest innovations in ionization technologies.  In addition to Electrospray Ionization (ESI), Atmospheric Pressure Photo Ionization (APPI), Atmospheric Pressure Chemical Ionization (APCI) and mixed mode (ESCi^®) techniques, the Universal Ion Source Architecture also supports Atmospheric Pressure Solids Analysis (ASAP) and Atmospheric Pressure Gas Chromatography (APGC) methods.

MALDI SYNAPT™ Systems combine the speed and simplicity of MALDI with ion mobility-based separations and high-resolution mass spectrometry to deliver enhanced specificity for the analysis of the most complex mixtures and challenging discovery applications.

SYNAPT G2-S is also compatible with third party interfaces, including DESI (Prosalia), DART (IonSense), LDTD(Phytronix) and TriVersa NanoMate (Advion)

Get up and running quickly and the discoveries come even faster! Maximiz simplicity and automation throughout your entire analytical workflow with Engineered Simplicity™-  a design philosophy which streamlines even the most complex applications from sample preparation and system set-up to reporting results and sharing them across your organization.

Prepare - IntelliStart™Technology ensures your system is ready to run for experts and beginners alike, whether you utilize MS, UPLC^®/MS or nanoUPLC/MS.

Analyze - Waters Universal Ion Source Architecture, QuanTof™, StepWave™ Triwave^® UPLC/MS^E, FastDDA,and ETD technologies, will equip you with an entirely new level of quantitative and qualitative capability.

Interpret - Process, visualize, compare, and interpret the most complex data automatically. Then turn it into meaningful information quickly with Informatics Sftware that support both MS and High Definition MS™workflows across applications.

Decide - Generate reports, share results and archive information easily with Waters laboratory informatics. Make decisions faster and better than ever before.

Scientific success depends on the integration of key technologies into systems tailored for specific needs. Waters is able to combine best-in-class technologies in sample preparation, column chemistries, Ultra PerformanceLC^®, high resolution mass spectrometry, laboratory informatics, instrument services and applications support to deliver the system solutions and support expertise you help you maximize success in a wide range of scientific disciplines:

• Protein identification and quantification – Identity^E and Expression^E High Definition Proteomics systems and ProteinLynx Global SERVER™ Software.
• Biopharmaceutical characterization – BiopharmaLynx™ Software
• Small molecule/metabolite identification – MetaboLynx™ XS and MassFragment™ software
• Profiling of small molecules (e.g. metabonomics) – MarkerLynx™ XS Software
• Untargeted and targeted quantification/identification (e.g. screening) – QuanLynx™, TargetLynx™, ChromaLynx™, and POSITIVE software
• Imaging of molecules from biological and synthetic matrices – Waters Imaging software
• Advanced structural analysis of macromolecules (e.g. protein complexes, carbohydrates polymers) – DriftScope™ Mobility Environment Software
• Open Access – OpenLynx™ Software
• Advanced discovery with multidimensional SYNAPT^® HDMS™ data – DriftScope 2.3, MS^E and HDMS^E viewer tool (MassLynx™ Software), ProteinLynx Global SERVER v2.5, BiopharmaLynx v1.3.