Quantitative Mass Spectrometry For Translational Research

Improve Biomedical Research and Evaluate Biomarkers More Efficiently and Effectively

One of the challenges of translating biomarkers from discovery to clinical utility is the availability of reliable quantitative assays that have the required sensitivity, precision and throughput to evaluate the biological variability of biomarkers in large sample studies. LC-MS is now becoming established as the credible alternative to immunoassays for quantitation of protein and small molecule biomarkers and offers superior analytical performance for biomarker studies.

Verifying biomarkers with traditional immunoassays often suffers from irreproducibility due to antibody non-specificity and lot-to-lot variability of antibodies. An MS-based approach provides a highly accurate and precise alternative workflow with lower assay development costs and shorter timelines. Simultaneous quantitation of multiple biomarkers in limited sample volumes empowers a deeper understanding of biological activity, as well as a more efficient evaluation of biomarkers. By using the gold standard technology for quantitative analysis, biomarker validation and verification studies will benefit from reduced analytical variability, enabling you to evaluate your biomarkers more efficiently and effectively.

Waters Xevo Mass Spectrometry Solutions Integrate:

  • Chromatographic systems and mass spectrometers that combine accurate reproducible separations and maximum MS performance with intelligent, easy-to-use laboratory software converting scientific data into useful information.
  • Novel micro-fluidics inlet that brings ultra sensitivity protein and peptide capability to the routine laboratory.
  • Chemistry consumables that work with your system to maximize sensitivity, increase throughput and ensure the development of robust methods.
  • Waters Applications Support and Global Services partner with you to optimize your laboratory efficiency, help maintain system peak performance, address scientific application challenges and meet your compliance requirements to ensure the success of your laboratory and business

 

Hear from our customers

Weqas - When you set reference standards, you need a gold-standard measuring tool

Peptides and Proteins are Not Small Molecules; Why Treat Them the Same?

Waters has vast experience in developing quantitative protein and peptide methods on LC-MS platforms. Download the peptide and protein application notebook to find out more. 

Application Notes

Peptide and Protein Bioanalysis Application Notebook

ionKey Micro-Fluidics Combine Ultra Sensitivity and Robustness for Protein Analysis

The Proteomics and Metabolomics Core Facility at Duke University, USA, uses a targeted proteomics method to quantify TAA or important proteins known to be involved in tumour activity that are targets for therapies.

Using Waters’ ionKey /MS technology to quantify tumor-associated antigen, this video shows the value of precise and accurate biomarker analysis by LC-MS. 

Scientists at a leading pharmaceutical company were able to reliably measure protein biomarkers related to heart failure using a combination of immunoaffinity clean up and the unique ionKey micro-fluidic inlet system.

Research Article

Development and Validation of an IA-LC/MS Method to Quantitate Active and Total B-Type Natriuretic Peptide in Human Plasma

Application Notes

Register and View On-Demand Webinar - Advancing Translational Research with LC-MS Innovations

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ionKey/MS System

Monitoring of Monoclonal Antibody Based Drugs

Monoclonal antibody (mAbs) are an increasingly important class of drugs that require measurement in bioanalytical studies and clinical research applications. LC-MS enables monitoring of mAbs with a specificity and repeatability previously unattainable using traditional immunoassay methods. Waters ProteinWorks kit ensures consistent digestion and sample pre-treatment for every sample.

Poster

Moving Towards the Standardization of Protein Quantification Workflows and Improving Their Analytical Reproducibility

Learn more about ProteinWorks

Analysis of Aldosterone for Clinical Research

Aldosterone is measured to study hypertension and is also a valuable biomarker for early stage drug development. The requirement to measure Aldosterone at low pg/mL is a challenge for many immunoassay based methods.

The performance of Xevo TQ-XS tandem quadrupole MS has been shown to provide quantitation down to 2 pg/mL aldosterone using only 200 µL of a serum sample. Even at low concentrations, Xevo TQ-XS provides the sensitivity required for the analysis of plasma aldosterone in clinical research settings enabling you to confidently quantify.

Poster

How Low Can We Go? Analysis of Aldosterone Using a Highly Analytically Sensitive Tandem Quadrupole Mass Spectrometer for Clinical Research

Application Note

Highly Analytically Sensitive Analysis of Aldosterone in Plasma on the Xevo TQ-XS for Clinical Research

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Xevo TQ-XS Tandem Quadrupole Mass Spectrometer

Analysis of Oxylipins in Clinical Research

Oxylipins are signalling molecules that play a vital role in inflammation processes and are therefore measured in many areas of disease and biomedical research. The ability to semi-quantitatively profile a wide range of oxylipin in plasma samples could help our understanding of their roles in health and disease, as well as serve as biomarkers for disease diagnosis or prognosis.

LC-MS methods for Oxylipins offer improved precision, specificity and the ability to quantify several Oxylipins in a single assay. The combination of solid-phase extraction and UPLC/ESI-tandem quadrupole mass spectrometry provides a comprehensive analysis of oxylipins in a dedicated analytical workflow.

Poster

Targeted SPE-UPLC-MS/MS Analysis of Oxylipins : From Profiling to Quantification for Translational Research Studies

Application Note

Targeted Lipidomics of Oxylipins (Oxygenated Fatty Acids)

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Xevo TQ-XS Tandem Quadrupole Mass Spectrometer

 

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