Peptide SEC Columns

Size-Exclusion Chromatography

Size exclusion, also known as gel filtration, chromatography separates proteins based on their sizes (hydrodynamic radii) in solution and not by absolute molecular weight with larger sized species eluting before smaller peptides.  The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column.   SEC columns containing comparatively larger pore size particles are selected as the molecular weight of the peptides of interest increase.

As with other bioseparations, use smaller particle size, UPLC/UHPLC offerings provide superior component resolution and in less time compared to our HPLC-based columns for this demanding application.

 

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SEC Optimization SEC Optimization Guide

Helpful considerations for robust UPLC-based SEC protein separations.
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