Oligonucleotide Separation Technology (OST) columns contain second-generation hybrid-silica BEH Technology™ particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography.
This chemistry delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.
The availability of 1.7 μm UPLC® technology particles in various column dimensions gives you the flexibility to meet various lab-scale isolation or analysis needs.
ACQUITY UPLC® OST C18, 1.7 µm columns (designed for use with an ACQUITY UPLC System) are well suited for the analyiss of detritylated oligonucleotides using ion-pair, reversed-phase chromatography. As indicated in the figure below, separations are comparable to that obtained by capillary gel electrophoresis (CGE) in terms of component resolution, yet analyses times are significantly decreased using Waters UPLC® Technology. The ability to resolve large oligonucleotide sequences (e.g., N from N-1) is possible due to the enhanced resolving power obtained using sub-3 µm, BEH Technology™ particles. In addition, quantitation with molecular weight characterization of the separated target oligonucleotide product form failure sequences is possible using Waters OST columns with hyphenated-Mass Spectrometry methods and MS friendly eluents.
Separation of Detritylated Oligodeoxythymidine Ladders by Capillary Gel Electrophoresis (CGE) vs. Ion-Pair, Reversed-Phase Chromatography