Column Advisor


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  • Method Attributes

    Compound retention and compound loading are two important attributes that help decide which column will be most useful for your separation.  The properties and attributes listed here help us recommend a column or column family for you based on the method attributes that you consider being important.  Choosing a column based on retention is analyte specific; the column choice is tailored to the majority of the compounds of interest.  However, some methods require columns that are tolerant to high mass loading conditions.

  • Compound Retention

    Chromatographic sorbents are chosen for their ability to retain a certain class or mixture of compounds.  In most cases, using a column that increases retention will allow you to increase the separation between closely eluting chromatographic peaks.  The columns in this section can be chosen by how well they can retain compound classes as well as those that are suited for polar compound retention.

  • Polar Analytes (RP)

    Polar compounds often elute in the void when organic modifiers are used in the mobile phase making accurate quantification difficult or impossible. It is common to use 100% aqueous mobile phases to retain polar compounds, such as metabolites or small organic acids.  The columns listed here are designed to maximize retention under these aqueous mobile phase conditions.

    134529162,134529189,
    • XSelect HSS T3 The best option for transferring methods from HPLC to ACQUITY UPLC. The XSelect HSS T3 columns are available in both HPLC and ACQUITY UPLC particle sizes.
    • Atlantis T3 The best option for reversed-phase HPLC methods and general purpose chromatography.
  • Polar Analytes (HILIC)

    HILIC (Hydrophilic Interaction Chromatography) is a technique that is commonly used when reversed-phase columns, such as the Atlantis T3, do not provide enough compound retention, even with 100% aqueous mobile phases.

    134528948,134529159,134828125,
    • XBridge Amide The extended pH range of this column is ideally suited for general purpose retention of polar acidic, polar basic or mixtures of compounds. Optimized for separating simple sugars (e.g.: sucrose, glucose) and polysaccharides.
    • Atlantis HILIC This silica based sorbent is the first choice for retaining small polar bases. It is best suited for low pH mobile phases. Available in HPLC particle sizes only.
    • CORTECS HILIC This high efficiency solid-core silica column is designed for retention of extremely polar, basic, water-soluble analytes. It is usable under low pH conditions.
  • General Mixtures

    Columns are recommended based on their ability to provide the widest retention capability for polar and non-polar compound mixtures using standard reversed-phase mobile phases.

    134529162,134529189,134828122,
    • XSelect HSS T3 The best option for when you desire to transfer methods from HPLC to UPLC in the future. The XSelect HSS T3 columns are available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • CORTECS C18 The best general purpose option when high-efficiency separations are desired. This solid-core column offers a balanced retention of acidic, basic, and neutral analytes, while providing enhanced resolution for complex mixtures.
    • Atlantis T3 The best option for HPLC methods development and general purpose chromatography. Available in HPLC particle sizes only.
  • Non-Polar Basic Analytes

    My recommendation is to use a general purpose column that is capable of using a wide mobile phase pH range, which gives the user the greatest ability to influence compound retention by modifying additive choice.  For example, the general rule for a basic analyte:
    - Low pH mobile phases decrease the retention of bases
    - High pH mobile phases increase the retention of bases

    134528929,134529153,134828118,
    • XBridge BEH C18 This column will give you the maximum retention for basic compounds when using high pH mobile phase buffers. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • XSelect CSH C18 This column is preferred when the majority of your methods are developed for low pH mobile phases. Out of these selections, this column will provide the best peak shape for bases at low pH. Available in both HPLC and ACQUITY UPLC particle sizes.
    • CORTECS C18+ Use this column for high-efficiency separations at low pH. The charged surface of the particle enables excellent peak shape of basic compounds at low pH, especially in the presence of low ionic strength modified mobile phases.
  • Non-Polar Acidic Analytes

    Retaining non-polar acidic compounds is most achievable using specialized phases other than C18; however, reproducibility and column lifetimes have been the two biggest concerns for users.  Generally, these columns are used with low pH mobile phases.

    134528929,134528947,134529178,
    • XSelect CSH Fluoro-Phenyl This unique fluorinated ligand provides the best retention for highly acidic compounds (i.e. strong acids) at low pH.
    • XBridge BEH Shield RP18 Generally less retentive for charged species compared to the XSelect Fluoro-Phenyl column, this column does well for weak acids and is more retentive that a standard alkyl C18 column
    • XBridge BEH C18 Even though this is the least retentive option, this column provides the longest lifetime at low pH. It is for this reason we recommend it as the best option for routine LC/MS applications.
  • Compound Loading

    Compound loading ability is an indicator of how well a chromatographic sorbent can tolerate extreme mass loading of an analyte before resolution or peak shape is compromised.  High load capabilities are important in areas such as impurity profiling and natural product isolation and are valued by preparative and dissolution scientists.

  • Column Attributes

    In some circumstances you will need a column to perform based on its chemical properties to improve analyte-stationary phase interactions. The column properties listed here are the most commonly referred to characteristics that aid chromatographic separations.

  • Selectivity

    Selectivity refers to the extent to which a column can separate compounds in mixtures without interferences from other components.  In general, most methods are developed using a classical C18 phase.  More recent phases, such as those containing embedded polar groups, provide alternative selectivity, while providing similar retention.

  • Stability

    The stability of a chromatographic sorbent refers to its ability to resist chemical attack from the mobile phase under a variety of operating conditions.  Chemical attack, including ligand loss through hydrolysis and particle dissolution, results in reduced column lifetime, peak deterioration, and changes in analyte selectivity.

  • Specific Requirement...

    Some columns are designed to have characteristics that can be applied to common application needs, such as general purpose LC/MS columns or those specific to biomolecular analysis.

The Column Advisor recommends the most appropriate columns depending on the application requirements you need to consider or the column characteristics you are looking for. Click on the appropriate tab and move your mouse on the left hand side table to reveal the corresponding application or column characteristics in the middle of the screen.

 
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Method Requirements

  • CSH / HSS Particle...

    Waters is on the forefront of modern HPLC columns.  Using industry-leading BEH, CSH, and HSS particle technology, our HPLC products eliminate time and cost per sample from your analytical process—while improving the quality of laboratory results. As one of the world’s primary column manufacturers, our ability to control column to column reproducibility, as well as produce some of the most innovative new chemistries, is second to none in the industry.

     

     

     

  • XSelect Columns

    XSelect CSH HPLC columns provide you with alternate selectivity compared to other commercially available reversed-phase columns. The high efficiency of the XSelect CSH column family provides seamless transferability between particle size and column formats (UPLC-HPLC-Preparative scale) without compromising peak shape and loading capacity for basic compounds.

  • XSelect CSH C18

    XSelect CSH C18 columns can be used as general purpose columns that provide alternative selectivity compared to other C18 sorbents. Charged Surface Hybrid (CSH) technology helps you overcome column related problems such as peak shape asymmetry and poor loading for basic compounds, column bleed, and slow mobile-phase re-equilibration.

    134528903,134529005,134529054,134529058,134529100,
  • XSelect CSH Fluoro-Phenyl

    Designed to maximize selectivity differences, the XSelect CSH Fluoro-Phenyl column provides you with maximum method development freedom, especially when using low pH mobile phases.  The non-encapped sorbent provides you with enhanced retention of acidic compounds.

    134528910,134529027,134529038,
  • XSelect CSH Phenyl-Hexyl

    XSelect CSH Phenyl-Hexyl columns provide you with alternative analyte selectivity compared to traditional reversed-phase columns and are a valuable tool for method development.  The trifunctionally bonded C6 phenyl-ligand is a robust, low bleed sorbent that selectively retains polyaromatic compounds through pi-pi interactions.

    134529027,
  • XSelect HSS C18

    The HSS C18 packing is the most stable commercially available bonded phase and does not suffer from peak shape issues that plague older stationary phases that rely upon steric protection (and lack of endcapping) to produce stability at low pH.  The HSS C18 HPLC columns are fully scalable and are completely transferable to UPLC separations.

    134529005,134529044,
  • XSelect HSS C18 SB

    HSS C18 SB columns are intended for low pH separations that contain complex mixtures of basic and non-basic compounds.  HSS C18 SB columns give you alternative analyte selectivity compared to most modern, high coverage, fully endcapped C18 stationary phases. HSS C18 SB HPLC columns are fully scalable and transferable to UPLC separations.

    134529016,134529038,
  • XSelect HSS T3

    The trifunctional alkyl C18 bonded phase used for the HSS T3 sorbents is compatible with 100% aqueous mobile phase and provides ultra-low MS bleed, while promoting superior polar compound retention. HSS T3 HPLC columns are fully scalable and transferable to UPLC separations.

    134528895,134528901,134529016,134529054,
  • XSelect HSS CN

    The XSelect HSS CN chemistry is an ultra-performance general purpose propyl cyano bonded phase that provides superior peak shape for bases, increased retention, and extremely long column lifetimes at low pH.  XSelect HSS CN HPLC columns are fully scalable and transferable to ACQUITY UPLC separations.

    134529027,134529038,134529044,
  • XSelect HSS PFP

    The XSelect HSS PFP is a non-endcapped silica-based HPLC column designed for low pH separations that require alternative selectivity compared to a fully endcapped, high coverage C18 phase.  XSelect HSS PFP HPLC columns are fully scalable and transferable to ACQUITY UPLC separations.

    134529027,134529038,
  • BEH Particles

    Waters is on the forefront of modern HPLC columns.  Using industry-leading BEH, CSH, and HSS particle technology, our HPLC products eliminate time and cost per sample from your analytical process—while improving the quality of laboratory results. As one of the world’s primary column manufacturers, our ability to control column to column reproducibility, as well as produce some of the most innovative new chemistries, is second to none in the industry.

     

     

     

  • XBridge Columns

    XBridge HPLC columns are designed for maximum flexibility in HPLC method development. You can achieve robust separations in less time and with greater confidence, while using the full range of mobile phase pH.  Industry leading manufacturing and product quality give you the most trusted columns for your most demanding chromatographic separations.

  • XBridge BEH C18

    As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12).  The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed.

    134528903,134528910,134528988,134529005,134529044,134529048,134529054,134529058,134529062,
  • XBridge Peptide BEH300 C1...

    The XBridge BEH300 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations.  The wide-pore (300Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

    134529062,
  • XBridge Peptide BEH130 C1...

    The XBridge BEH130 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations.  The small-pore (130Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

    134529062,
  • XBridge Protein BEH300 C4...

    Waters Protein Separation Technology (PrST) columns are QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point.  If you compare traditional to C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery and improve peak capacity. 

    134529062,
  • XBridge Oligonucleotide B...

    Waters Oligonucleotide Separation Technology (OST) columns are used to separate detritylated synthetic oligonucleotides based on ion-pair, reversed-phase chromatography.  Each column is QC tested using Waters MassPrep OST standards to ensure repeatable performance for your most demanding oligonucleotide assays

    134529062,
  • XBridge BEH Shield RP18

    XBridge BEH Shield RP18 columns complement the XBridge BEH C18 and C8 phases and provide a necessary and powerful tool for HPLC method development. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds compared to straight chain alkyl columns.

    134528910,134529016,
  • XBridge BEH Phenyl

    XBridge BEH Phenyl columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds. This most chemically stable commercially available Phenyl sorbent on the market and provides you with ultra-low column bleed, long column lifetimes and excellent peak shape.

    134529027,134529048,
  • XBridge BEH HILIC

    XBridge BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes.

    134529068,
  • XBridge BEH Amide

    XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars.  XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa.

    134528908,134529068,
  • Solid-core Particl...

    TBA

  • CORTECS Columns

    Achieve faster separations and improve sample throughput without sacrificing HPLC/UHPLC separation performance. CORTECS HPLC Columns offer the unique advantage of operating at lower back pressure and higher flow rates without sacrificing efficiency or resolution. Compared to a fully porous particle, the CORTECS particle provides unique characteristics and innovative charged surface modifications that are suitable for wide variety of applications.

  • CORTECS C18

    The C18 ligand is the most popular choice in method development because of its stability and ability to retain a variety of analytes. CORTECS C18 is a traditional C18 solid-core bonded phase which exhibits high efficiency with a balanced retention of acids, bases and neutrals at low- and mid-range pH. It provides superb resolution and retention for complex mixtures.

    134528901,134529005,134529044,
  • CORTECS C18+

    CORTECS C18+ is a general purpose, high efficiency, solid-core, reversed-phase column which features a positively charged surface modification. Charged-surface technology utilizes a controlled, low-level surface charge for enhanced selectivity and exceptional peak shape for basic compounds when using acidic, low-ionic strength mobile phases such as formic acid.

    134528903,134529054,134529058,134529100,
  • CORTECS HILIC

    CORTECS HILIC Columns are designed specifically for the retention of very polar compounds using HILIC methods of separation. CORTECS HILIC Columns features an unbonded CORTECS solid-core silica particle that offers high efficiency under low pH conditions.

    134528908,134529068,
  • Silica Particles

    .

  • Atlantis Columns

    The Atlantis family of HPLC columns provides leading performance for polar compound retention, while maintaining versatility, reproducibility and accuracy in quantitative bioanalytical assays.  Atlantis reversed-phase and HILIC columns are chosen to improve chromatographic retention of moderately polar to very polar analytes and metabolites.

  • Atlantis T3

    Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules and should be your first choice when developing separations of polar and non-polar compounds. 

    134528895,134528901,
  • Atlantis HILIC

    Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography.   HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

    134528908,134529068,
  • Sunfire Columns

    SunFire C18 HPLC columns set the standard for state-of-the-art silica bonding are often selected for their resolving power and reliability to meet both analytical and preparative method needs.  For general purpose use, SunFire Columns exhibit high efficiency, excellent peak shape, and unmatched batch-to-batch reproducibility.

  • SunFire C18

    SunFire C18 columns are used for general purpose method development at low pH.  The C18 ligand is highly retentive, especially for basic compounds and it is ideally suited for purification and impurity profile assays.

    134528903,134528988,134529058,134529100,
  • CSH / HSS Particle...

    Waters is on the forefront of modern HPLC columns.  Using industry-leading BEH, CSH, and HSS particle technology, our HPLC products eliminate time and cost per sample from your analytical process—while improving the quality of laboratory results. As one of the world’s primary column manufacturers, our ability to control column to column reproducibility, as well as produce some of the most innovative new chemistries, is second to none in the industry.

     

     

     

  • XSelect Columns

    XSelect CSH HPLC columns provide you with alternate selectivity compared to other commercially available reversed-phase columns. The high efficiency of the XSelect CSH column family provides seamless transferability between particle size and column formats (UPLC-HPLC-Preparative scale) without compromising peak shape and loading capacity for basic compounds.

  • XSelect CSH C18

    XSelect CSH C18 columns can be used as general purpose columns that provide alternative selectivity compared to other C18 sorbents. Charged Surface Hybrid (CSH) technology helps you overcome column related problems such as peak shape asymmetry and poor loading for basic compounds, column bleed, and slow mobile-phase re-equilibration.

    134528903,134529005,134529054,134529058,134529100,
  • XSelect CSH Fluoro-Phenyl

    Designed to maximize selectivity differences, the XSelect CSH Fluoro-Phenyl column provides you with maximum method development freedom, especially when using low pH mobile phases.  The non-encapped sorbent provides you with enhanced retention of acidic compounds.

    134528910,134529027,134529038,
  • XSelect CSH Phenyl-Hexyl

    XSelect CSH Phenyl-Hexyl columns provide you with alternative analyte selectivity compared to traditional reversed-phase columns and are a valuable tool for method development.  The trifunctionally bonded C6 phenyl-ligand is a robust, low bleed sorbent that selectively retains polyaromatic compounds through pi-pi interactions.

    134529027,
  • XSelect HSS C18

    The HSS C18 packing is the most stable commercially available bonded phase and does not suffer from peak shape issues that plague older stationary phases that rely upon steric protection (and lack of endcapping) to produce stability at low pH.  The HSS C18 HPLC columns are fully scalable and are completely transferable to UPLC separations.

    134529005,134529044,
  • XSelect HSS C18 SB

    HSS C18 SB columns are intended for low pH separations that contain complex mixtures of basic and non-basic compounds.  HSS C18 SB columns give you alternative analyte selectivity compared to most modern, high coverage, fully endcapped C18 stationary phases. HSS C18 SB HPLC columns are fully scalable and transferable to UPLC separations.

    134529016,134529038,
  • XSelect HSS T3

    The trifunctional alkyl C18 bonded phase used for the HSS T3 sorbents is compatible with 100% aqueous mobile phase and provides ultra-low MS bleed, while promoting superior polar compound retention. HSS T3 HPLC columns are fully scalable and transferable to UPLC separations.

    134528895,134528901,134529016,134529054,
  • XSelect HSS CN

    The XSelect HSS CN chemistry is an ultra-performance general purpose propyl cyano bonded phase that provides superior peak shape for bases, increased retention, and extremely long column lifetimes at low pH.  XSelect HSS CN HPLC columns are fully scalable and transferable to ACQUITY UPLC separations.

    134529027,134529038,134529044,
  • XSelect HSS PFP

    The XSelect HSS PFP is a non-endcapped silica-based HPLC column designed for low pH separations that require alternative selectivity compared to a fully endcapped, high coverage C18 phase.  XSelect HSS PFP HPLC columns are fully scalable and transferable to ACQUITY UPLC separations.

    134529027,134529038,
  • BEH Particles

    Waters is on the forefront of modern HPLC columns.  Using industry-leading BEH, CSH, and HSS particle technology, our HPLC products eliminate time and cost per sample from your analytical process—while improving the quality of laboratory results. As one of the world’s primary column manufacturers, our ability to control column to column reproducibility, as well as produce some of the most innovative new chemistries, is second to none in the industry.

     

     

     

  • XBridge Columns

    XBridge HPLC columns are designed for maximum flexibility in HPLC method development. You can achieve robust separations in less time and with greater confidence, while using the full range of mobile phase pH.  Industry leading manufacturing and product quality give you the most trusted columns for your most demanding chromatographic separations.

  • Solid-core Particl...

    TBA

  • CORTECS Columns

    Achieve faster separations and improve sample throughput without sacrificing HPLC/UHPLC separation performance. CORTECS HPLC Columns offer the unique advantage of operating at lower back pressure and higher flow rates without sacrificing efficiency or resolution. Compared to a fully porous particle, the CORTECS particle provides unique characteristics and innovative charged surface modifications that are suitable for wide variety of applications.

  • Silica Particles

    .

  • Atlantis Columns

    The Atlantis family of HPLC columns provides leading performance for polar compound retention, while maintaining versatility, reproducibility and accuracy in quantitative bioanalytical assays.  Atlantis reversed-phase and HILIC columns are chosen to improve chromatographic retention of moderately polar to very polar analytes and metabolites.

  • Sunfire Columns

    SunFire C18 HPLC columns set the standard for state-of-the-art silica bonding are often selected for their resolving power and reliability to meet both analytical and preparative method needs.  For general purpose use, SunFire Columns exhibit high efficiency, excellent peak shape, and unmatched batch-to-batch reproducibility.

The Column Advisor recommends the most appropriate columns depending on the application requirements you need to consider or the column characteristics you are looking for. Click on the appropriate tab and move your mouse on the left hand side table to reveal the corresponding application or column characteristics in the middle of the screen.

  • Method Attributes

    Compound retention and compound loading are two important attributes that help decide which column will be most useful for your separation.  The properties and attributes listed here help us recommend a column or column family for you based on the method attributes that you consider being important.  Choosing a column based on retention is analyte specific; the column choice is tailored to the majority of the compounds of interest.  However, some methods require columns that are tolerant to high mass loading conditions.

  • Compound Retention

    Chromatographic sorbents are chosen for their ability to retain a certain class or mixture of compounds.  In most cases, using a column that increases retention will allow you to increase the separation between closely eluting chromatographic peaks.  The columns in this section can be chosen by how well they can retain compound classes as well as those that are suited for polar compound retention.

  • Polar Analytes (RP)

    Polar compounds often elute in the void when organic modifiers are used in the mobile phase making accurate quantification difficult or impossible. It is common to use 100% aqueous mobile phases to retain polar compounds, such as metabolites or small organic acids.  The columns listed here are designed to maximize retention under these aqueous mobile phase conditions.

    134529162,134529189,
    • XSelect HSS T3 The best option for transferring methods from HPLC to ACQUITY UPLC. The XSelect HSS T3 columns are available in both HPLC and ACQUITY UPLC particle sizes.
    • Atlantis T3 The best option for reversed-phase HPLC methods and general purpose chromatography.
  • Polar Analytes (HILIC)

    HILIC (Hydrophilic Interaction Chromatography) is a technique that is commonly used when reversed-phase columns, such as the Atlantis T3, do not provide enough compound retention, even with 100% aqueous mobile phases.

    134528948,134529159,134828125,
    • XBridge Amide The extended pH range of this column is ideally suited for general purpose retention of polar acidic, polar basic or mixtures of compounds. Optimized for separating simple sugars (e.g.: sucrose, glucose) and polysaccharides.
    • Atlantis HILIC This silica based sorbent is the first choice for retaining small polar bases. It is best suited for low pH mobile phases. Available in HPLC particle sizes only.
    • CORTECS HILIC This high efficiency solid-core silica column is designed for retention of extremely polar, basic, water-soluble analytes. It is usable under low pH conditions.
  • General Mixtures

    Columns are recommended based on their ability to provide the widest retention capability for polar and non-polar compound mixtures using standard reversed-phase mobile phases.

    134529162,134529189,134828122,
    • XSelect HSS T3 The best option for when you desire to transfer methods from HPLC to UPLC in the future. The XSelect HSS T3 columns are available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • CORTECS C18 The best general purpose option when high-efficiency separations are desired. This solid-core column offers a balanced retention of acidic, basic, and neutral analytes, while providing enhanced resolution for complex mixtures.
    • Atlantis T3 The best option for HPLC methods development and general purpose chromatography. Available in HPLC particle sizes only.
  • Non-Polar Basic Analytes

    My recommendation is to use a general purpose column that is capable of using a wide mobile phase pH range, which gives the user the greatest ability to influence compound retention by modifying additive choice.  For example, the general rule for a basic analyte:
    - Low pH mobile phases decrease the retention of bases
    - High pH mobile phases increase the retention of bases

    134528929,134529153,134828118,
    • XBridge BEH C18 This column will give you the maximum retention for basic compounds when using high pH mobile phase buffers. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • XSelect CSH C18 This column is preferred when the majority of your methods are developed for low pH mobile phases. Out of these selections, this column will provide the best peak shape for bases at low pH. Available in both HPLC and ACQUITY UPLC particle sizes.
    • CORTECS C18+ Use this column for high-efficiency separations at low pH. The charged surface of the particle enables excellent peak shape of basic compounds at low pH, especially in the presence of low ionic strength modified mobile phases.
  • Non-Polar Acidic Analytes

    Retaining non-polar acidic compounds is most achievable using specialized phases other than C18; however, reproducibility and column lifetimes have been the two biggest concerns for users.  Generally, these columns are used with low pH mobile phases.

    134528929,134528947,134529178,
    • XSelect CSH Fluoro-Phenyl This unique fluorinated ligand provides the best retention for highly acidic compounds (i.e. strong acids) at low pH.
    • XBridge BEH Shield RP18 Generally less retentive for charged species compared to the XSelect Fluoro-Phenyl column, this column does well for weak acids and is more retentive that a standard alkyl C18 column
    • XBridge BEH C18 Even though this is the least retentive option, this column provides the longest lifetime at low pH. It is for this reason we recommend it as the best option for routine LC/MS applications.
  • Compound Loading

    Compound loading ability is an indicator of how well a chromatographic sorbent can tolerate extreme mass loading of an analyte before resolution or peak shape is compromised.  High load capabilities are important in areas such as impurity profiling and natural product isolation and are valued by preparative and dissolution scientists.

  • Acidic Analytes

    For acidic analytes, the best chromatographic performance under high loading conditions is achieved using low pH mobile phases and columns that are designed for chemical stability under these conditions.

    134529153,134529172,
    • XSelect CSH C18 Provides alternative selectivity to the SunFire C18 column. This new hybrid-based column also is suitable for high load performance and has been optimized for low pH separations. Available in both HPLC and ACQUITY UPLC particle sizes.
    • SunFire C18 This silica-based column provides superior loading and lifetime under low pH conditions. Available in HPLC and preparative formats only.
  • Basic Compounds

    For basic analytes, there are two ways to maintain separation and peak shape under high loading conditions.  For the highest analyte loading for basic compounds, many users prefer to use high pH mobile phases.  The high pH mobile ensures that the analyte is in a neutral charge state, thus maximizing reversed-phase retention and reducing any unwanted ionic interactions with the sorbent substrate. When the compound is chemically unstable at high pH, it is common to use ion-pairing agents and low mobile phase pH conditions. 

    134528929,134529153,134529172,
    • XSelect CSH C18 Often selected for low pH applications without the need for unnecessary mobile phase additives, such as TFA. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • XBridge BEH C18 Selected for high pH applications because the highest mass loading for bases is often obtained when the compound is eluted in its neutral state at high pH. Available in both HPLC and ACQUITY UPLC particle sizes.
    • SunFire C18 A silica-based option that provides excellent loading and alternative selecivity for bases at low pH. Available in HPLC and preparative formats only.
  • Column Attributes

    In some circumstances you will need a column to perform based on its chemical properties to improve analyte-stationary phase interactions. The column properties listed here are the most commonly referred to characteristics that aid chromatographic separations.

  • Selectivity

    Selectivity refers to the extent to which a column can separate compounds in mixtures without interferences from other components.  In general, most methods are developed using a classical C18 phase.  More recent phases, such as those containing embedded polar groups, provide alternative selectivity, while providing similar retention.

  • Classic C18 Behavior

    Columns that exhibit C18-like selectivity are classified using the USP designation L1, and are often referred to as “ODS” phases.  These general purpose columns are widely used to solve the most common problems for reversed-phase separations.

    134528929,134529184,134828122,
    • CORTECS C18 The best general purpose option when high-efficiency separations are desired. This solid-core column offers a balanced retention of acidic, basic, and neutral analytes, while providing enhanced resolution for complex mixtures.
    • XBridge BEH C18 The best choice for applications that exclusively use high pH mobile phases. An excellent high pH choice for high column lifetimes.
    • XSelect HSS C18 This classic silica-based option that provides alternative selectivity for users who desire a general purpose C18 phase suitable for low pH applications.
  • Specialty C18

    These columns are classified with a USP L1 designation; however, they contain specialized ligand bonding or endcapping that gives preferential selectivity to a particular compound class. These columns include embedded polar (shield) columns and columns designed for polar retention. 

    134528947,134529187,134529189,
    • XBridge BEH Shield RP18 Contains a polar embedded group within the C18 ligand that provides alternative selectivity and sharper peaks compared to an alkyl C18, such as XBridge C18 (e.g., increased retention for phenolic compounds).
    • XSelect HSS C18 SB This column contains a standard silica-based alkyl C18 ligand that is non-endcapped exposing active silanol sites, which increases interactions for basic analytes.
    • XSelect HSS T3 The premier column for reversed-phase polar compound retention.
  • Alternate to C18

    Columns that contain alternative ligands have markedly different selectivity when compared to standard reversed-phase C18 columns.  They are ideally suited for method development, where it is desirable to discriminate between critical pairs or to significantly increase the separation of chromatographic interferences.

    134529036,134529178,134529181,134636982,134637004,
    • XSelect HSS PFP A silica-based perfluorinated phenyl column designed to maximize selectivity and retention for polar and non-polar basic compounds. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • XSelect HSS CN This column chemistry can be used for multiple modes of chromatographic separation, including reversed-phase, normal-phase and HILIC separations. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms.
    • XSelect CSH Fluoro-Phenyl Designed to maximize selectivity differences, for acidic compounds. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms. (USP L43)
    • XSelect CSH Phenyl-Hexyl XSelect CSH Phenyl-Hexyl columns (USP L11) provide you with alternative analyte selectivity for polyaromatic compounds through pi-pi interactions. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transferbetween LC platforms.
    • XBridge Phenyl These columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds. Available in both HPLC and ACQUITY UPLC particle sizes for easy method transfer between LC platforms. (USP L11)
  • Non Endcapped Columns

    A non-endcapped stationary phase can provide different analyte selectivity compared to one that is endcapped, although sometimes at the expense of chromatographic peak shape and pH stability.  A non-endcapped stationary phase exposes un-bonded silanol groups for potential interaction with the analyte.  Such chemistries are valuable method development tools.

    134529178,134529187,134636982,134637004,
    • XSelect HSS C18 SB This column contains a standard silica-based alkyl C18 ligand that is non-endcapped exposing active silanol sites. This column is categorized as a USP L1 column.
    • XSelect HSS PFP This silica-based column contains perfluorophenyl ligand that is non-endcapped. This column is categorized as a USP L43 column.
    • XSelect CSH Fluoro-Phenyl This column is an alternative to the traditional C18 column and is classified as USP L43 column. The non-encapped fluorinated phase provides alternative selectivity to standard LC separations.
    • XSelect HSS CN This column is an alternative to the traditional C18 column and is classified as USP L10 column. The non-encapped cyano phase provides alternative selectivity to standard LC separations.
  • Stability

    The stability of a chromatographic sorbent refers to its ability to resist chemical attack from the mobile phase under a variety of operating conditions.  Chemical attack, including ligand loss through hydrolysis and particle dissolution, results in reduced column lifetime, peak deterioration, and changes in analyte selectivity.

  • Low pH

    Column lifetime performance using mobile phases with pH less than 3 can be controlled by optimizing ligand density and bonding.  Under these acidic conditions a tri-functionally bonded ligand and chemically stable base particle will outperform a mono- or di- functionally bonded stationary phase.

    134528929,134529184,134828122,
    • XSelect HSS C18 This column is selected when you require a silica-based reversed-phase column option for alternative selectivity.
    • XBridge BEH C18 Is the most modern option available for general purpose LC separations at low pH. The nature of the hybrid-based particle used in this column has reduced silanol activity compared to a similar silica-based C18 column.
    • CORTECS C18 The best general purpose option when high-efficiency separations are desired. This solid-core column offers a balanced retention of acidic, basic, and neutral analytes, while providing enhanced resolution for complex mixtures.
  • High pH

    Column lifetime performance is often compromised when using mobile phases that exceed pH 7 and most silica based columns begin to degrade rapidly beyond pH 8.  Hybrid based particles with high ligand densities exhibit the longest lifetimes and provide the ability to routinely use mobile phases that exceed pH 10 for extended periods.

    134528929,134529036,
    • XBridge C18 The most modern option available for general purpose LC separations using high pH mobile phases.
    • XBridge BEH Phenyl This column is selected when you require an alternative to XBridge C18. The highly stable phenyl ligand provides complimentary selectivity especially for polyaromatic compounds.
  • Specific Requirement...

    Some columns are designed to have characteristics that can be applied to common application needs, such as general purpose LC/MS columns or those specific to biomolecular analysis.

  • LC/MS Recommended

    Most modern LC columns are compatible with MS instrumentation; however, column bleed can compromise MS performance.  It is for this reason that following columns are selected for their mechanical and chemical stability under a wide range of operating conditions.

    134528929,134529189,134828118,
    • XBridge BEH C18 Provides the widest usable pH range and the best column lifetimes for general purpose LC/MS work. These columns are ideally suited for volatile buffers between pH 2 and 11.
    • CORTECS C18+ Use this column for high-efficiency separations at low pH. The charged surface of the particle enables excellent peak shape of basic compounds at low pH, especially in the presence of low ionic strength modified mobile phases.
    • XSelect HSS T3 These columns are selected when you require a silica-based column option for alternative selectivity. These columns are designed to be used for both polar and non polar acid, neutral and basic compounds.
  • Efficiency

    Column efficiency or plate count measures peak dispersion. A highly efficient column will generate chromatograms with tall, sharp peaks and enhance resolution, speed and sensitivity. The following high performance columns utitilize solid-core technology, which provides an efficiency advantage when compared to similar columns of a fully porous nature.

    134828118,134828122,134828125,
    • CORTECS C18+ Use this column for high-efficiency separations at low pH. The charged surface of the particle enables excellent peak shape of basic compounds at low pH, especially in the presence of low ionic strength modified mobile phases.
    • CORTECS C18 The best general purpose option when high-efficiency separations are desired. This solid-core column offers a balanced retention of acidic, basic, and neutral analytes, while providing enhanced resolution for complex mixtures.
    • CORTECS HILIC This high efficiency solid-core silica column is designed for retention of extremely polar, basic, water-soluble analytes. It is usable under low pH conditions.
  • Best Peakshape for Bases

    The best performing and most desirable columns maintain good peak shape for basic compounds under a wide set of operating conditions, including the use of reduced ionic-strength mobile phases (e.g: 0.1% formic acid).  These columns will also maintain this symmetrical peak shape as the analyte mass load in increased.

    134529153,134529172,134828118,
    • XSelect CSH C18 These columns are specifically designed for use with non-buffered, low ionic strength mobile phases. The controlled positive charge on the CSH particle reduces the effect of high mass load and is the best choice for when you need to scale the separation
    • CORTECS C18+ Use this column for high-efficiency separations at low pH. The charged surface of the particle enables excellent peak shape of basic compounds at low pH, especially in the presence of low ionic strength modified mobile phases.
    • SunFire C18 These columns are selected when you require a silica-based column. The proprietary bonding and endcapping used for SunFire columns provides symmetrical peak shapes and improved resolution for acidic, neutral and basic compounds at low to intermediate pH
  • Biomolecule and Wide Pore

    These columns are design and QC tested for biomolecular separations, specifically protein and peptides using reversed-phase conditions.  These columns have wider pore volumes making them more suitable for these larger molecule separations.

    134528936,134528937,134528938,134631577,
    • XBridge Peptide BEH300 C18 The XBridge BEH300 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations.
    • XBridge Protein BEH300 C4 Waters Protein Separation Technology (PrST) columns are QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point.
    • XBridge Peptide BEH130 C18 The XBridge BEH130 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations.
    • XBridge Oligonucleotide BEH C18 Waters Oligonucleotide Separation Technology (OST) columns are used to separate detritylated synthetic oligonucleotides based on ion-pair, reversed-phase chromatography.
  • HILIC

    HILIC (Hydrophilic Interaction Chromatography) is a secondary technique that is commonly used when specialized reversed-phase polar retentions columns, such as the Atlantis T3, do not provide enough compound retention, even with 100% aqueous mobile phases.
    Polar stationary phases, such as unbonded silica are typically used with polar solvents such as acetonitrile to achieve separation of polar analytes.  Stronger retention is observed when the polarity of the stationary increases (hybrid particle versus silica particle) and the polarity of the mobile phase decreases (methanol versus acetonitrile).

    134528948,134528949,134529159,134828125,
    • Atlantis HILIC This silica based sorbent is the first choice for retaining small polar bases; however, it is limited to low pH mobile phases. Available in HPLC formats only.
    • CORTECS HILIC This high efficiency solid-core silica column is designed for retention of extremely polar, basic, water-soluble analytes. It is usable under low pH conditions.
    • XBridge BEH Amide The first choice for sugar and carbohydrate analysis. It is also recommended for retaining polar acids and neutral polar compound mixtures. Available in HPLC and UPLC formats.
    • XBridge BEH HILIC This hybrid based sorbent has less HILIC retention than the Atlantis HILIC. It is used when you require more pH stability under more aggressive mobile phases conditions (pH range: 1-9). Available in HPLC and UPLC formats for easy method transfer.
The product recommendations presented here are intended to provide universal guidelines that may deliver successful separations in the shortest amount of time. Please note that these proposals are based upon known product performance for certain classes of molecules under a known set of conditions and do not guarantee a successful separation.